Genomic organization of the Shc-related phosphotyrosine adapters and characterization of the full-length Sck/ShcB: Specific association of p68-Sck/ShcB with pp135

Takuya Kojima, Yoshiaki Yoshikawa, Shinji Takada, Masahiro Sato, Takeshi Nakamura, Naoki Takahashi, Neal G. Copeland, Debra J. Gilbert, Nancy A. Jenkins, Nozomu Mori

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

The Shc gene family is an emerging family, containing at least three members designated Shc/ShcA, Sck/Sli/ShcB, N-Shc/Rai/ShcC in mammals. In this study, we determined the genomic organization of the mouse Shc family. Coding regions of ShcA, B, and C each comprised 12 exons, spanned approximately 6, 20, and 65 kb, and located on chromosome 3, 10, and 13, respectively. Based on this genome analysis, we determined the full-length structure of mouse Sck/ShcB as a 68-kD protein. We found that the 68-kD full-length Sck/ShcB was more efficiently phosphorylated upon EGF treatment than the previously-analyzed CH2-deleted form. We also found that Sck specifically interacted with a 135-kD phosphoprotein (pp135) through its SH2 domain following membrane depolarization. The Sck-pp135 interaction was reduced by Src kinase inhibitors. These results suggest that Sck, but not N-Shc nor Shc, transmit signals in conjunction with pp135 following Src activation and/or calcium entry in the cell.

Original languageEnglish (US)
Pages (from-to)1039-1047
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume284
Issue number4
DOIs
StatePublished - 2001

Keywords

  • Adapter
  • Genome
  • N-Shc
  • Phosphotyrosine
  • PTB
  • Sck
  • SH2
  • ShcB
  • ShcC
  • Signal transduction
  • Src

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Fingerprint Dive into the research topics of 'Genomic organization of the Shc-related phosphotyrosine adapters and characterization of the full-length Sck/ShcB: Specific association of p68-Sck/ShcB with pp135'. Together they form a unique fingerprint.

Cite this