Genomic organization, chromosomal localization, tissue distribution, and biophysical characterization of a novel mammalian Shaker-related voltage- gated potassium channel, Kvl.7

Katalin Kalman, Angela Nguyen, Julie Tseng-Crank, Iain D. Dukes, Grischa Chandy, Carolyn M. Hustad, Neal G. Copeland, Nancy A. Jenkins, Harvey Mohrenweiser, Brigitte Brandriff, Michael Cahalan, George A. Gutman, K. George Chandy

Research output: Contribution to journalArticle

60 Scopus citations

Abstract

We report the isolation of a novel mouse voltage-gated Shaker-related K+ channel gene, Kvl. 7 (Kcna7/KCNA7). Unlike other known Kv1 family genes that have intronless coding regions, the protein-coding region of Kv1.7 is interrupted by a 1.9-kilobase pair intron. The Kv1.7 gene and the related Kv3.3 (Kcnc3/KCNC3) gene map to mouse chromosome 7 and human chromosome 19q13.3, a region that has been suggested to contain a diabetic susceptibility locus. The mouse Kv1.7 channel is voltage-dependent and rapidly inactivating, exhibits cumulative inactivation, and has a single channel conductance of 21 pS. It is potently blocked by noxiustoxin and stichodactylatoxin, and is insensitive to tetraethylammonium, kaliotoxin, and charybdotoxin. Northern blot analysis reveals ~3-kilobase pair Kv1.7 transcripts in mouse heart and skeletal muscle. In situ hybridization demonstrates the presence of Kv1.7 in mouse pancreatic islet cells. Kv1.7 was also isolated from mouse brain and hamster insulinoma cells by polymerase chain reaction.

Original languageEnglish (US)
Pages (from-to)5851-5857
Number of pages7
JournalJournal of Biological Chemistry
Volume273
Issue number10
DOIs
StatePublished - Mar 6 1998

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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