TY - JOUR
T1 - Genomic organization and chromosomal mapping of the mouse P-cadherin gene
AU - Hatta, Masayuki
AU - Miyatani, Seiji
AU - Copeland, Neal G.
AU - Gilbert, Debra J.
AU - Jenkins, Nancy A.
AU - Takeichi, Masatoshi
N1 - Funding Information:
We thank Martin Ringwald and Rolf Kemler for the E-cadherin (uvomorulin) probe, Debbie Swing, Denise Angle and Brian Cho for excellent technical assistance, and Glenn Radice for critical comments on the manuscript. This work was supported by research grants (no. 02258102 and 02262216) from the Ministry of Education, Science and Culture of Japan to M.T., and, in part, by the National Cancer institute, DHHS, under contract NO1-CO-74101 with ABL.
PY - 1991/8/25
Y1 - 1991/8/25
N2 - Cadherins are a family of Ca2+-dependent cell adhesion molecules, that includes P-cadherin, E-cadherin, N-cadherin and L-CAM. In this study, the genomic organization of the mouse P-cadherin gene was determined by analyzing overlapping DNA clones obtained from a mouse genomic library. The results showed that this gene spans over 45kb and consists of 15 exons. A marked feature of this gene is that the first intron is 23kbp long accounting for half its length. Comparisons of this structure with that of L-CAM, a chicken cadherin, revealed that the exon-intron boundaries are conserved between the two genes except that the P-cadherin first exon includes the correspoding first and second exons of the L-CAM gene. This gene was also similar to the other in that the second intron, which corresponds to the P-cadherin first intron, is exceptionally longer than other introns. These results suggest that the exon-intron pattern conserved in these genes is of significance for generation of domain structure of cadherin molecules or for their transcriptional regulation. We also determined the chromosomal localization of the P-cadherin gene by interspecific backcross analysis, and found that this gene is located in the central region of mouse chromosome 8 and linked with the E-cadherin locus. This is the first evidence for the linkage of different cadherin genes.
AB - Cadherins are a family of Ca2+-dependent cell adhesion molecules, that includes P-cadherin, E-cadherin, N-cadherin and L-CAM. In this study, the genomic organization of the mouse P-cadherin gene was determined by analyzing overlapping DNA clones obtained from a mouse genomic library. The results showed that this gene spans over 45kb and consists of 15 exons. A marked feature of this gene is that the first intron is 23kbp long accounting for half its length. Comparisons of this structure with that of L-CAM, a chicken cadherin, revealed that the exon-intron boundaries are conserved between the two genes except that the P-cadherin first exon includes the correspoding first and second exons of the L-CAM gene. This gene was also similar to the other in that the second intron, which corresponds to the P-cadherin first intron, is exceptionally longer than other introns. These results suggest that the exon-intron pattern conserved in these genes is of significance for generation of domain structure of cadherin molecules or for their transcriptional regulation. We also determined the chromosomal localization of the P-cadherin gene by interspecific backcross analysis, and found that this gene is located in the central region of mouse chromosome 8 and linked with the E-cadherin locus. This is the first evidence for the linkage of different cadherin genes.
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U2 - 10.1093/nar/19.16.4437
DO - 10.1093/nar/19.16.4437
M3 - Article
C2 - 1886768
AN - SCOPUS:0025880195
VL - 19
SP - 4437
EP - 4441
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 16
ER -