Genomic organization and chromosomal location of the mouse vasoactive intestinal polypeptide 1 (VPAC1) receptor

Hitoshi Hashimoto; Akiko Nishino; Norihito Shintani; Nami Hagihara; Neal G. Copeland; Nancy A. Jenkins; Kyohei Yamamoto; Toshio Matsuda; Takeshi Ishihara; Shigekazu Nagata; Akemichi Baba

doi:10.1006/geno.1999.5805

Genomic organization and chromosomal location of the mouse vasoactive intestinal polypeptide 1 (VPAC₁) receptor

Hitoshi Hashimoto, Akiko Nishino, Norihito Shintani, Nami Hagihara, Neal G. Copeland, Nancy A. Jenkins, Kyohei Yamamoto, Toshio Matsuda, Takeshi Ishihara, Shigekazu Nagata, Akemichi Baba

Research output: Contribution to journal › Article

22 Scopus citations

Abstract

The gene encoding the mouse vasoactive intestinal polypeptide type 1 (VPAC₁) receptor was cloned, and its structural organization was determined. The gene (Vipr1) is more than 16 kh in length and is divided into 13 exons. The 5'-flanking region is highly GC-rich and lacks an apparent TATA box, but contains a CCAAT box, three potential Sp1-binding sites, and two potential AP-2-binding sites. Promoter analysis of the 5'-flanking region of Vipr1 using a luciferase gene reporter system revealed that the isolated 5'- flanking region has functional promoter activity. The mouse Vipr1 gene is encoded by a single gene, which was mapped to the distal region of mouse chromosome 9. This region is syntenic with human chromosome 3p, where the human VPAC₁ receptor gene has been mapped.

Original language	English (US)
Pages (from-to)	90-93
Number of pages	4
Journal	Genomics
Volume	58
Issue number	1
DOIs	https://doi.org/10.1006/geno.1999.5805
State	Published - May 15 1999

ASJC Scopus subject areas

Genetics

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Genomic organization and chromosomal location of the mouse vasoactive intestinal polypeptide 1 (VPAC₁) receptor. / Hashimoto, Hitoshi; Nishino, Akiko; Shintani, Norihito; Hagihara, Nami; Copeland, Neal G.; Jenkins, Nancy A.; Yamamoto, Kyohei; Matsuda, Toshio; Ishihara, Takeshi; Nagata, Shigekazu; Baba, Akemichi.

In: Genomics, Vol. 58, No. 1, 15.05.1999, p. 90-93.

Research output: Contribution to journal › Article

Hashimoto, Hitoshi ; Nishino, Akiko ; Shintani, Norihito ; Hagihara, Nami ; Copeland, Neal G. ; Jenkins, Nancy A. ; Yamamoto, Kyohei ; Matsuda, Toshio ; Ishihara, Takeshi ; Nagata, Shigekazu ; Baba, Akemichi. / Genomic organization and chromosomal location of the mouse vasoactive intestinal polypeptide 1 (VPAC₁) receptor. In: Genomics. 1999 ; Vol. 58, No. 1. pp. 90-93.

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abstract = "The gene encoding the mouse vasoactive intestinal polypeptide type 1 (VPAC1) receptor was cloned, and its structural organization was determined. The gene (Vipr1) is more than 16 kh in length and is divided into 13 exons. The 5'-flanking region is highly GC-rich and lacks an apparent TATA box, but contains a CCAAT box, three potential Sp1-binding sites, and two potential AP-2-binding sites. Promoter analysis of the 5'-flanking region of Vipr1 using a luciferase gene reporter system revealed that the isolated 5'- flanking region has functional promoter activity. The mouse Vipr1 gene is encoded by a single gene, which was mapped to the distal region of mouse chromosome 9. This region is syntenic with human chromosome 3p, where the human VPAC1 receptor gene has been mapped.",

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AU - Yamamoto, Kyohei

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