TY - JOUR
T1 - Genomic organization and chromosomal localization of the human and mouse genes encoding the α receptor component for ciliary neurotrophic factor
AU - Valenzuela, David M.
AU - Rojas, Eduardo
AU - Le Beau, Michelle M.
AU - Espinosa, Rafael
AU - Brannan, Camilynn I.
AU - Mcclain, Joyce
AU - Masiakowski, Piotr
AU - Ip, Nancy Y.
AU - Copeland, Neal G.
AU - Jenkins, Nancy A.
AU - Yancopoulos, George D.
N1 - Funding Information:
We thank Dr. L. S. Schleifer and the entire Discovery Group at Regeneron for their enthusiastic support and insightful scientific input, as well as Dr. Mark E. Furth for important intellectual input at the beginning of this study. We thank Jennifer Griffith, Yuan Kong, Robert Carrol, Anthony A. Fernald, D. J. Gilbert, B. Cho, and D. A. Swing for excellent technical assistance. This work was supported in part by PHS Grant CA40046 (M.M.L.), the National Cancer Institute, DHHS, under Contract NOl-CO-74101 with ABL.
Copyright:
Copyright 2015 Elsevier B.V., All rights reserved.
PY - 1995/1/1
Y1 - 1995/1/1
N2 - Ciliary neurotrophic factor (CNTF) has recently been found to share receptor components with, and to be structurally related to, a family of broadly acting cytokines, including interleukin-6, leukemia inhibitory factor, and oncostatin M. However, the CNTF receptor complex also includes a CNTF-specific component known as CNTF receptor α (CNTFRα). Here we describe the molecular cloning of the human and mouse genes encoding CNTFR. We report that the human and mouse genes have an identical intron-exon structure that correlates well with the domain structure of CNTFRα. That is, the signal peptide and the immunoglobulin-like domain are each encoded by single exons, the cytokine receptor-like domain is distributed among 4 exons, and the C-terminal glycosyl phosphatidylinositol recognition domain is encoded by the final coding exon. The position of the introns within the cytokine receptor-like domain corresponds to those found in other members of the cytokine receptor superfamily. Confirming a recent study using radiation hybrids, we have also mapped the human CNTFR gene to chromosome band 9p13 and the mouse gene to a syntenic region of chromosome 4.
AB - Ciliary neurotrophic factor (CNTF) has recently been found to share receptor components with, and to be structurally related to, a family of broadly acting cytokines, including interleukin-6, leukemia inhibitory factor, and oncostatin M. However, the CNTF receptor complex also includes a CNTF-specific component known as CNTF receptor α (CNTFRα). Here we describe the molecular cloning of the human and mouse genes encoding CNTFR. We report that the human and mouse genes have an identical intron-exon structure that correlates well with the domain structure of CNTFRα. That is, the signal peptide and the immunoglobulin-like domain are each encoded by single exons, the cytokine receptor-like domain is distributed among 4 exons, and the C-terminal glycosyl phosphatidylinositol recognition domain is encoded by the final coding exon. The position of the introns within the cytokine receptor-like domain corresponds to those found in other members of the cytokine receptor superfamily. Confirming a recent study using radiation hybrids, we have also mapped the human CNTFR gene to chromosome band 9p13 and the mouse gene to a syntenic region of chromosome 4.
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U2 - 10.1016/0888-7543(95)80121-2
DO - 10.1016/0888-7543(95)80121-2
M3 - Article
C2 - 7774913
AN - SCOPUS:0028942171
VL - 25
SP - 157
EP - 163
JO - Genomics
JF - Genomics
SN - 0888-7543
IS - 1
ER -