Genomic confirmation of vancomycinresistant Enterococcus transmission from deceased donor to liver transplant recipient

Ali Bashir, Oliver Attie, Mitchell Sullivan, Robert Sebra, Kavindra V. Singh, Deena Altman, Theodore Pak, Jayeeta Dutta, Kieran Chacko, Elizabeth Webster, Martha Lewis, Camille Hamula, Kristin W.Delli Carpini, Barbara E. Murray, Andrew Kasarskis, Harm Van Bakel, Shirish Huprikar

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

In a liver transplant recipient with vancomycin-resistant Enterococcus (VRE) surgical site and bloodstream infection, a combination of pulsed-field gel electrophoresis, multilocus sequence typing, and whole genome sequencing identified that donor and recipient VRE isolates were highly similar when compared to time-matched hospital isolates. Comparison of de novo assembled isolate genomes was highly suggestive of transplant transmission rather than hospital-acquired transmission and also identified subtle internal rearrangements between donor and recipient missed by other genomic approaches. Given the improved resolution, whole-genome assembly of pathogen genomes is likely to become an essential tool for investigation of potential organ transplant transmissions.

Original languageEnglish (US)
Article numbere0170449
JournalPLoS ONE
Volume12
Issue number3
DOIs
StatePublished - Mar 2017

ASJC Scopus subject areas

  • General

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