Genomic confirmation of vancomycinresistant Enterococcus transmission from deceased donor to liver transplant recipient

Ali Bashir; Oliver Attie; Mitchell Sullivan; Robert Sebra; Kavindra V. Singh; Deena Altman; Theodore Pak; Jayeeta Dutta; Kieran Chacko; Elizabeth Webster; Martha Lewis; Camille Hamula; Kristin W.Delli Carpini; Barbara E. Murray; Andrew Kasarskis; Harm Van Bakel; Shirish Huprikar

doi:10.1371/journal.pone.0170449

Genomic confirmation of vancomycinresistant Enterococcus transmission from deceased donor to liver transplant recipient

Ali Bashir, Oliver Attie, Mitchell Sullivan, Robert Sebra, Kavindra V. Singh, Deena Altman, Theodore Pak, Jayeeta Dutta, Kieran Chacko, Elizabeth Webster, Martha Lewis, Camille Hamula, Kristin W.Delli Carpini, Barbara E. Murray, Andrew Kasarskis, Harm Van Bakel, Shirish Huprikar

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Abstract

In a liver transplant recipient with vancomycin-resistant Enterococcus (VRE) surgical site and bloodstream infection, a combination of pulsed-field gel electrophoresis, multilocus sequence typing, and whole genome sequencing identified that donor and recipient VRE isolates were highly similar when compared to time-matched hospital isolates. Comparison of de novo assembled isolate genomes was highly suggestive of transplant transmission rather than hospital-acquired transmission and also identified subtle internal rearrangements between donor and recipient missed by other genomic approaches. Given the improved resolution, whole-genome assembly of pathogen genomes is likely to become an essential tool for investigation of potential organ transplant transmissions.

Original language	English (US)
Article number	e0170449
Journal	PLoS ONE
Volume	12
Issue number	3
DOIs	https://doi.org/10.1371/journal.pone.0170449
State	Published - Mar 2017

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Bashir, A., Attie, O., Sullivan, M., Sebra, R., Singh, K. V., Altman, D., Pak, T., Dutta, J., Chacko, K., Webster, E., Lewis, M., Hamula, C., Carpini, K. W. D., Murray, B. E., Kasarskis, A., Van Bakel, H., & Huprikar, S. (2017). Genomic confirmation of vancomycinresistant Enterococcus transmission from deceased donor to liver transplant recipient. PLoS ONE, 12(3), Article e0170449. https://doi.org/10.1371/journal.pone.0170449

Bashir, A, Attie, O, Sullivan, M, Sebra, R, Singh, KV, Altman, D, Pak, T, Dutta, J, Chacko, K, Webster, E, Lewis, M, Hamula, C, Carpini, KWD, Murray, BE, Kasarskis, A, Van Bakel, H & Huprikar, S 2017, 'Genomic confirmation of vancomycinresistant Enterococcus transmission from deceased donor to liver transplant recipient', PLoS ONE, vol. 12, no. 3, e0170449. https://doi.org/10.1371/journal.pone.0170449

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abstract = "In a liver transplant recipient with vancomycin-resistant Enterococcus (VRE) surgical site and bloodstream infection, a combination of pulsed-field gel electrophoresis, multilocus sequence typing, and whole genome sequencing identified that donor and recipient VRE isolates were highly similar when compared to time-matched hospital isolates. Comparison of de novo assembled isolate genomes was highly suggestive of transplant transmission rather than hospital-acquired transmission and also identified subtle internal rearrangements between donor and recipient missed by other genomic approaches. Given the improved resolution, whole-genome assembly of pathogen genomes is likely to become an essential tool for investigation of potential organ transplant transmissions.",

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AU - Pak, Theodore

AU - Dutta, Jayeeta

AU - Chacko, Kieran

AU - Webster, Elizabeth

AU - Lewis, Martha

AU - Hamula, Camille

AU - Carpini, Kristin W.Delli

AU - Murray, Barbara E.

AU - Kasarskis, Andrew

AU - Van Bakel, Harm

AU - Huprikar, Shirish

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Genomic confirmation of vancomycinresistant Enterococcus transmission from deceased donor to liver transplant recipient

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