Genomic analysis of carbapenem-resistant Pseudomonas aeruginosa ST143 clone showing susceptibility to broad-spectrum cephalosporins

Ana Paula Streling, Rodrigo Cayô, Carolina S. Nodari, Luiz G.P. Almeida, Fernanda F. Santos, Blake Hanson, An Q. Dinh, Ana Tereza R. Vasconcelos, Willian R. Miller, Cesar A. Arias, Ana C. Gales

Research output: Contribution to journalComment/debatepeer-review

Abstract

Objectives: Using whole-genome sequencing (WGS), we aimed to characterise a Pseudomonas aeruginosa ST143 clinical strain (Pb9) that presented resistance to meropenem and imipenem and susceptibility to piperacillin/tazobactam and broad-spectrum cephalosporins. Methods: The antimicrobial susceptibility profile was confirmed by broth microdilution. WGS was performed using an Illumina MiSeq platform to identify possible genetic determinants of β-lactam resistance. Transcription levels of chromosomally encoded efflux systems and oprD were evaluated by RT-qPCR. Results: WGS analysis showed that no acquired carbapenemase-encoding gene was found in isolate Pb9, although mutations in the chromosomally encoded β-lactamase genes blaOXA-488, blaPIB-1 and blaPDC-5 were observed. In addition, we detected a premature stop codon in the major porin-encoding gene oprD coupled with hyperexpression of MexAB–OprM and MexEF–OprN. Conclusion: Our results suggest that the β-lactam resistance phenotype presented by strain Pb9 might be related to an association of OprD loss with hyperexpression of the efflux pump systems MexAB–OprM and MexEF–OprN. However, the contribution of OXA-488, PDC-5 and PIB-1 to this phenotype remains unclear and warrants further investigation.

Original languageEnglish (US)
Pages (from-to)177-179
Number of pages3
JournalJournal of Global Antimicrobial Resistance
Volume26
DOIs
StatePublished - Sep 2021

Keywords

  • Carbapenem resistance
  • Genome
  • Non-fermenting Gram-negative bacilli
  • OXA-50
  • PDC-5
  • Pseudomonas

ASJC Scopus subject areas

  • Microbiology
  • Immunology and Allergy
  • Immunology
  • Microbiology (medical)

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