TY - JOUR
T1 - Genome-wide significance for a modifier of age at neurological onset in Huntington's disease at 6q23-24
T2 - The HD MAPS study
AU - Li, Jian Liang
AU - Hayden, Michael R.
AU - Warby, Simon C.
AU - Durr, Alexandra
AU - Morrison, Patrick J.
AU - Nance, Martha
AU - Ross, Chirstopher A.
AU - Margolis, Russell L.
AU - Rosenblatt, Adam
AU - Squitieri, Ferdinando
AU - Frati, Luigi
AU - Gómez-Tortosa, Estrella
AU - García, Carmen Ayuso
AU - Suchowersky, Oksana
AU - Klimek, Mary Lou
AU - Trent, Ronald J A
AU - McCusker, Elizabeth
AU - Novelletto, Andrea
AU - Frontali, Marina
AU - Paulsen, Jane S.
AU - Jones, Randi
AU - Ashizawa, Tetsuo
AU - Lazzarini, Alice
AU - Wheeler, Vanessa C.
AU - Prakash, Ranjana
AU - Xu, Gang
AU - Djoussé, Luc
AU - Mysore, Jayalakshmi Srinidhi
AU - Gillis, Tammy
AU - Hakky, Michael
AU - Cupples, L. Adrienne
AU - Saint-Hilaire, Marie H.
AU - Cha, Jang Ho J
AU - Hersch, Steven M.
AU - Penney, John B.
AU - Harrison, Madaline B.
AU - Perlman, Susan L.
AU - Zanko, Andrea
AU - Abramson, Ruth K.
AU - Lechich, Anthony J.
AU - Duckett, Ayana
AU - Marder, Karen
AU - Conneally, P. Michael
AU - Gusella, James F.
AU - MacDonald, Marcy E.
AU - Myers, Richard H.
PY - 2006/8/17
Y1 - 2006/8/17
N2 - Background: Age at onset of Huntington's disease (HD) is correlated with the size of the abnormal CAG repeat expansion in the HD gene; however, several studies have indicated that other genetic factors also contribute to the variability in HD age at onset. To identify modifier genes, we recently reported a whole-genome scan in a sample of 629 affected sibling pairs from 295 pedigrees, in which six genomic regions provided suggestive evidence for quantitative trait loci (QTL), modifying age at onset in HD. Methods: In order to test the replication of this finding, eighteen microsatellite markers, three from each of the six genomic regions, were genotyped in 102 newly recruited sibling pairs from 69 pedigrees, and data were analyzed, using a multipoint linkage variance component method, in the follow-up sample and the combined sample of 352 pedigrees with 753 sibling pairs. Results: Suggestive evidence for linkage at 6q23-24 in the follow-up sample (LOD = 1.87, p = 0.002) increased to genome-wide significance for linkage in the combined sample (LOD = 4.05, p = 0.00001), while suggestive evidence for linkage was observed at 18q22, in both the follow-up sample (LOD = 0.79, p = 0.03) and the combined sample (LOD = 1.78, p = 0.002). Epistatic analysis indicated that there is no interaction between 6q23-24 and other loci. Conclusion: In this replication study, linkage for modifier of age at onset in HD was confirmed at 6q23-24. Evidence for linkage was also found at 18q22. The demonstration of statistically significant linkage to a potential modifier locus opens the path to location cloning of a gene capable of altering HD pathogenesis, which could provide a validated target for therapeutic development in the human patient.
AB - Background: Age at onset of Huntington's disease (HD) is correlated with the size of the abnormal CAG repeat expansion in the HD gene; however, several studies have indicated that other genetic factors also contribute to the variability in HD age at onset. To identify modifier genes, we recently reported a whole-genome scan in a sample of 629 affected sibling pairs from 295 pedigrees, in which six genomic regions provided suggestive evidence for quantitative trait loci (QTL), modifying age at onset in HD. Methods: In order to test the replication of this finding, eighteen microsatellite markers, three from each of the six genomic regions, were genotyped in 102 newly recruited sibling pairs from 69 pedigrees, and data were analyzed, using a multipoint linkage variance component method, in the follow-up sample and the combined sample of 352 pedigrees with 753 sibling pairs. Results: Suggestive evidence for linkage at 6q23-24 in the follow-up sample (LOD = 1.87, p = 0.002) increased to genome-wide significance for linkage in the combined sample (LOD = 4.05, p = 0.00001), while suggestive evidence for linkage was observed at 18q22, in both the follow-up sample (LOD = 0.79, p = 0.03) and the combined sample (LOD = 1.78, p = 0.002). Epistatic analysis indicated that there is no interaction between 6q23-24 and other loci. Conclusion: In this replication study, linkage for modifier of age at onset in HD was confirmed at 6q23-24. Evidence for linkage was also found at 18q22. The demonstration of statistically significant linkage to a potential modifier locus opens the path to location cloning of a gene capable of altering HD pathogenesis, which could provide a validated target for therapeutic development in the human patient.
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U2 - 10.1186/1471-2350-7-71
DO - 10.1186/1471-2350-7-71
M3 - Article
C2 - 16914060
AN - SCOPUS:33749416817
SN - 1471-2350
VL - 7
JO - BMC Medical Genetics
JF - BMC Medical Genetics
M1 - 71
ER -