TY - JOUR
T1 - Genome-Wide analysis and proteomic studies reveal APE1/Ref-1 multifunctional role in mammalian cells
AU - Vascotto, Carlo
AU - Cesaratto, Laura
AU - Zeef, Leo A.H.
AU - Deganuto, Marta
AU - Dambrosio, Chiara
AU - Scaloni, Andrea
AU - Romanello, Milena
AU - Damante, Giuseppe
AU - Taglialatela, Giulio
AU - Delneri, Daniela
AU - Kelley, Mark R.
AU - Mitra, Sankar
AU - Quadrifoglio, Franco
AU - Tell, Gianluca
PY - 2009/2
Y1 - 2009/2
N2 - Apurinic apyrimidinic endonuclease/redox effector factor 1 (APE1/Ref-1) protects cells from oxidative stress by acting as a central enzyme in base excision repair pathways of DNA lesions and through its independent activity as a redox transcriptional co-activator. Dysregulation of this protein has been associated with cancer development. At present, contrasting data have been published regarding the biological relevance of the two functions as well as the molecular mechanisms involved. Here, we combined both mRNA expression profiling and prote- omic analysis to determine the molecular changes associated with APE1 loss-of-expression induced by siRNA technology. This approach identified a role of APE1 in cell growth, apop- tosis, intracellular redox state, mitochondrial function, and cytoskeletal structure. Overall, our data show that APE1 acts as a hub in coordinating different and vital functions in mammalian cells, highlighting the molecular determinants of the multifunctional nature of APE1 protein.
AB - Apurinic apyrimidinic endonuclease/redox effector factor 1 (APE1/Ref-1) protects cells from oxidative stress by acting as a central enzyme in base excision repair pathways of DNA lesions and through its independent activity as a redox transcriptional co-activator. Dysregulation of this protein has been associated with cancer development. At present, contrasting data have been published regarding the biological relevance of the two functions as well as the molecular mechanisms involved. Here, we combined both mRNA expression profiling and prote- omic analysis to determine the molecular changes associated with APE1 loss-of-expression induced by siRNA technology. This approach identified a role of APE1 in cell growth, apop- tosis, intracellular redox state, mitochondrial function, and cytoskeletal structure. Overall, our data show that APE1 acts as a hub in coordinating different and vital functions in mammalian cells, highlighting the molecular determinants of the multifunctional nature of APE1 protein.
KW - APE1
KW - Apoptosis
KW - Oxidative stress
KW - Ref-1
KW - SiRNA
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U2 - 10.1002/pmic.200800638
DO - 10.1002/pmic.200800638
M3 - Article
C2 - 19180539
AN - SCOPUS:61549109882
SN - 1615-9853
VL - 9
SP - 1058
EP - 1074
JO - Proteomics
JF - Proteomics
IS - 4
ER -