Genetic identification of cryptic genospecies of Haemophilus causing urogenital and neonatal infections by PCR using specific primers targeting genes coding for 16S rRNA

Roland Quentin, Raymond Ruimy, Agnes Rosenau, James M. Musser, Richard Christen

Research output: Contribution to journalArticle

44 Scopus citations

Abstract

Previous genetic analysis of Haemophilus influenzae strains isolated from genital and neonatal infections identified a group of biotype IV that constitutes a cryptic genospecies only distantly related to H. influenzae and H. haemolyticus. Small-subunit rRNA genes of two representative strains of this genital Haemophilus genospecies (strains 16N and 2406) were sequenced. The analysis indicated that these strains form a monophyletic unit with H. haemolyticus and H. influenzae biogroups Influenzae and Aegyptius and are more closely related to H. haemolyticus than to H. influenzae biogroups Influenzae and Aegyptius. 16S rRNA gene sequences were used to formulate primers for PCR-based identification of cryptic genital Haemophilus organisms. A 242-bp fragment was amplified from strains belonging to the genital Haemophilus genospecies but not from strains of 12 other Haemophilus species, including strains of H. influenzae biotype IV sensu stricto.

Original languageEnglish (US)
Pages (from-to)1380-1385
Number of pages6
JournalJournal of Clinical Microbiology
Volume34
Issue number6
DOIs
StatePublished - Jun 1996

ASJC Scopus subject areas

  • Microbiology (medical)

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