Genetic diversity among type emm28 group a Streptococcus strains causing invasive infections and pharyngitis

Nicole M. Green, Stephen B. Beres, Edward A. Graviss, James E. Allison, Allison J. McGeer, Jaana Vuopio-Varkila, Ranee B. LeFebvre, James M. Musser

Research output: Contribution to journalArticlepeer-review

46 Scopus citations


Genome sequencing of group A Streptococcus (GAS) has revealed that prophages account for the vast majority of gene content differences between strains. Serotype M28 strains are a leading cause of pharyngitis and invasive infections, but little is known about genetic diversity present in natural populations of these organisms. To study this issue, population-based samples of 568 strains from Ontario, Canada; Finland; and Houston, Texas, were analyzed. Special attention was given to analysis of variation in prophage-encoded virulence gene content by a PCR-based method. Thirty and 29 distinct prophage-encoded virulence gene profiles were identified among pharyngitis and invasive infection isolates. Thirteen profiles, representing the majority of the strains, were shared between these two classes of isolates. Significant differences were observed in the frequency of occurrence of certain prophage toxin gene profiles and infection type. M28 strains are highly diverse in prophage-encoded virulence gene content and integration site, supporting the key concept that prophages are critical contributors to GAS genetic diversity and population biology. Nucleotide sequence variation in the emm gene (encodes M protein) was also examined. Only three allelic variants were identified in the hypervariable portion of the emm28 gene. All but one strain had the same inferred amino acid sequence in the first 100 amino acids of the mature M28 protein. In contrast, size differences in the emm28 gene and inferred protein due to variable numbers of C-terminal repeats were common. The presence of macrolide resistance genes (mefA, ermB, and ermTR) was analyzed by PCR, and less than 2% of the strains were positive.

Original languageEnglish (US)
Pages (from-to)4083-4091
Number of pages9
JournalJournal of Clinical Microbiology
Issue number8
StatePublished - Aug 2005

ASJC Scopus subject areas

  • Microbiology (medical)


Dive into the research topics of 'Genetic diversity among type emm28 group a Streptococcus strains causing invasive infections and pharyngitis'. Together they form a unique fingerprint.

Cite this