TY - JOUR
T1 - Genetic complementation of a glucocorticoid receptor deficiency by expression of cloned receptor cDNA
AU - Miesfeld, Roger
AU - Rusconi, Sandro
AU - Godowski, Paul J.
AU - Maler, Bonnie A.
AU - Okret, Sam
AU - Wikström, Ann Charlotte
AU - Gustafsson, Jan Åke
AU - Yamamoto, Keith R.
PY - 1986/8/1
Y1 - 1986/8/1
N2 - We isolated and sequenced 6.3 kb of cDNA encoding the rat glucocorticoid receptor, a protein that binds and activates a class of hormone-dependent transcriptional enhancers. Receptor-containing cells produce receptor mRNAs of ≅6.5 kb and ≅4.8 kb that differ only in their 3′ nontranslated regions; an open reading frame of 795 amino acids resides within the 5′ portion of the transcripts. The coding region was expressed in vitro, in transient transfections, and in stable transfectants of a receptor-deficient cell line. The protein products are indistinguishable from bona fide receptor with respect to sedimentation and electrophoretic mobility, antibody reactivity, and hormone and DNA binding. Moreover, the cloned receptor protein activates its corresponding enhancers, restoring to the receptor-deficient cells the full capacity for regulated enhancement.
AB - We isolated and sequenced 6.3 kb of cDNA encoding the rat glucocorticoid receptor, a protein that binds and activates a class of hormone-dependent transcriptional enhancers. Receptor-containing cells produce receptor mRNAs of ≅6.5 kb and ≅4.8 kb that differ only in their 3′ nontranslated regions; an open reading frame of 795 amino acids resides within the 5′ portion of the transcripts. The coding region was expressed in vitro, in transient transfections, and in stable transfectants of a receptor-deficient cell line. The protein products are indistinguishable from bona fide receptor with respect to sedimentation and electrophoretic mobility, antibody reactivity, and hormone and DNA binding. Moreover, the cloned receptor protein activates its corresponding enhancers, restoring to the receptor-deficient cells the full capacity for regulated enhancement.
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U2 - 10.1016/0092-8674(86)90659-8
DO - 10.1016/0092-8674(86)90659-8
M3 - Article
C2 - 3755378
AN - SCOPUS:0022458855
VL - 46
SP - 389
EP - 399
JO - Cell
JF - Cell
SN - 0092-8674
IS - 3
ER -