Genetic ablation of a mouse gene expressed specifically in brain

D. M. Kingsley, E. M. Rinchik, L. B. Russell, H. P. Ottiger, J. G. Sutcliffe, N. G. Copeland, N. A. Jenkins

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

The 1B1075 gene was initially identified from a cDNA clone of a rat brain messenger RNA expressed in particular subsets of CNS neurons and pituitary cells. Although the protein encoded by this gene is of unknown function, its sequence suggests that it may be related to secretogranin proteins, which are found in association with secretory granules in a variety of peptidergic endocrine and neuronal cells. Here we show that the mouse 1B1075 gene is located between the dilute (d) and short ear (se) genes on chromosome 9. Many different deletion mutations have previously been isolated in the genetic region that includes these genes. By producing mice carrying two deletions that overlap at the 1B1075 locus, the gene for this brain-specific message can be completely eliminated from otherwise viable animals. The animals missing the 1B1075 gene provide an important new tool for determining the function of this gene in the brain. In addition, these results provide a new molecular entry point for detailed characterization of other genes in the d-se region.

Original languageEnglish (US)
Pages (from-to)395-399
Number of pages5
JournalEMBO Journal
Volume9
Issue number2
DOIs
StatePublished - 1990

Keywords

  • Chromosome mapping
  • Gene ablation
  • Mouse mutation
  • Neurogenetics

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Fingerprint Dive into the research topics of 'Genetic ablation of a mouse gene expressed specifically in brain'. Together they form a unique fingerprint.

Cite this