Abstract
BACKGROUND. Interleukin-17 receptor-like protein (IL-17RL) expressed in prostate tissues changes with advanced cancers due to extensive alternative splicing, which affects the final protein. Predominant IL-17RL splice isoform variants have not been identified, hindering functional studies. METHODS. A cDNA library of IL-17RL transcripts was arrayed onto nylon membranes. Individual transcript exon structures were determined by successively probing membranes with exon-specific oligonucleotides. The most common variants were transiently over-expressed in 293T cells. RESULTS. We detected >90 different IL-17RL isoforms. Three most abundant isoforms account for approximately half the total transcripts; the full-length variant just over 11%. Surprisingly, most alternative splicing does not alter the reading frame of the full-length molecule; therefore, resulting proteins vary mostly in N-terminal domains. CONCLUSIONS. IL-17RL exists as multiple isoforms due to extensive alternative splicing. We identified the most abundant splices in prostate tissue and established a technique to investigate changes in RNA IL-17RL splicing that occur in advanced cancers.
Original language | English (US) |
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Pages (from-to) | 1268-1274 |
Number of pages | 7 |
Journal | Prostate |
Volume | 66 |
Issue number | 12 |
DOIs | |
State | Published - Sep 1 2006 |
Keywords
- Alternative splicing
- Gleason grade
- IL-17RC
- IL-17RL
- Prostate cancer
ASJC Scopus subject areas
- Oncology
- Urology