Generation of an Fgf9 conditional null allele

Yongshun Lin, Guoqin Liu, Fen Wang

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

The fibroblast growth factor (FGF) family consists of 22 widely expressed regulatory polypeptides and controls a broad spectrum of cellular processes. Accumulating data show that FGF9 plays important roles both in embryogenesis and in adult tissue homeostasis. Ablation of Fgf9 alleles leads to lethality at the neonatal stage mainly due to malformations of the lung, as well as causing male-to-female sex reversal. To circumvent the neonatal lethality resulting from disruption of the Fgf9 gene, which hinders further characterization of the role of FGF9 in adult tissue function and homeostasis, we generated an Fgf9 conditional null allele for spatiotemporal- and tissue-specific disruption of Fgf9. Using gene targeting in mouse embryonic stem (ES) cells, we introduced two loxP sites flanking exon 1 in the Fgf9 allele, which encodes 93 amino acid residues at the N-terminal of FGF9. Our results indicate that the Fgf9 conditional null allele is a true conditional null that encodes wild type activity and reverts to a null allele after recombination mediated by the Cre recombinase.

Original languageEnglish (US)
Pages (from-to)150-154
Number of pages5
JournalGenesis
Volume44
Issue number3
DOIs
StatePublished - Mar 2006

Keywords

  • Cre-LoxP
  • Fibroblast growth factors
  • Gene targeting
  • Prostate

ASJC Scopus subject areas

  • Genetics
  • Endocrinology
  • Cell Biology

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