Gene trap screening for cell surface and extracellular matrix molecules produced by chondrocytes

Shin Ichi Shirasawa, Masayuki Yoshimi, Hideaki Kamochi, Takeshi Muneta, James H. Kimura, Magnus Höök, Tamayuki Shinomura

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

We have developed a simple and unique strategy for identifying cell surface and extracellular matrix molecules produced by chondrocytes. Our strategy comprises two methods, retrovirus-based signal sequence gene trapping and culturing of Swarm rat chondrosarcoma chondrocytes. After infection with a retrovirus vector and isolation of hygromycin-resistant clones, trapped genes could be easily identified by the 5′ rapid amplification of cDNA ends (5′ RACE) method. Furthermore, the treatment of isolated clones with gadolinium chloride enabled us to determine whether the trapped gene expression was dependent on the state of chondrogenic differentiation.

Original languageEnglish (US)
Pages (from-to)79-85
Number of pages7
JournalJournal of Biochemistry
Volume137
Issue number1
DOIs
StatePublished - Jan 2005

Keywords

  • Chondrocytes
  • Dedifferentiation
  • Gadolinium chloride
  • Gene trap
  • Signal sequence

ASJC Scopus subject areas

  • Biochemistry

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