TY - JOUR
T1 - Gene expression profiling of chemically induced rat bladder tumors
AU - Yao, Ruisheng
AU - Yi, Jun
AU - Grubbs, Clinton J.
AU - Lubet, Ronald A.
AU - You, Ming
PY - 2007/3
Y1 - 2007/3
N2 - A variety of genetic alterations and gene expression changes are involved in the pathogenesis of bladder tumors. To explore expression changes in 4-hydroxybutyl(butyl)nitrosamine-induced rat bladder tumors, microarray analysis was performed. Analysis yielded 1,138 known genes and 867 expressed sequence tags that were changed when comparing tumors to normal rat epithelia. Altered genes included cell cycle-related genes, EGFR-Ras signaling genes, apoptosis genes, growth factors, and oncogenes. Using the pathway visualization tool GenMAPP, we found that these genes can be grouped along several pathways that control apoptosis, cell cycle, and integrin-mediated cell adhesion. When comparing current data with previous mouse bladder tumor data, we found that > 280 of the same known genes were differentially expressed in both mouse and rat bladder tumors, including cell cycle-related genes, small G proteins, apoptosis genes, oncogenes, tumor-suppressor genes, and growth factors. These results suggest that multiple pathways are involved in rat bladder tumorigenesis, and a common molecular mechanism was found in both rat and mouse bladder tumors.
AB - A variety of genetic alterations and gene expression changes are involved in the pathogenesis of bladder tumors. To explore expression changes in 4-hydroxybutyl(butyl)nitrosamine-induced rat bladder tumors, microarray analysis was performed. Analysis yielded 1,138 known genes and 867 expressed sequence tags that were changed when comparing tumors to normal rat epithelia. Altered genes included cell cycle-related genes, EGFR-Ras signaling genes, apoptosis genes, growth factors, and oncogenes. Using the pathway visualization tool GenMAPP, we found that these genes can be grouped along several pathways that control apoptosis, cell cycle, and integrin-mediated cell adhesion. When comparing current data with previous mouse bladder tumor data, we found that > 280 of the same known genes were differentially expressed in both mouse and rat bladder tumors, including cell cycle-related genes, small G proteins, apoptosis genes, oncogenes, tumor-suppressor genes, and growth factors. These results suggest that multiple pathways are involved in rat bladder tumorigenesis, and a common molecular mechanism was found in both rat and mouse bladder tumors.
KW - Chemical carcinogen
KW - Expression profile
KW - Microarray
KW - Pathways
KW - Rat bladder tumors
UR - http://www.scopus.com/inward/record.url?scp=33947375580&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33947375580&partnerID=8YFLogxK
U2 - 10.1593/neo.06814
DO - 10.1593/neo.06814
M3 - Article
C2 - 17401461
AN - SCOPUS:33947375580
VL - 9
SP - 207
EP - 221
JO - Neoplasia (United States)
JF - Neoplasia (United States)
SN - 1522-8002
IS - 3
ER -