GDNF overexpression fails to provoke muscle recovery from botulinum toxin poisoning: A preliminary study

Kenny F. Lin; Hank H. Sun; Matthew R. MacEwan; Susan E. MacKinnon; Philip J. Johnson

doi:10.1002/micr.21967

GDNF overexpression fails to provoke muscle recovery from botulinum toxin poisoning: A preliminary study

Kenny F. Lin, Hank H. Sun, Matthew R. MacEwan, Susan E. MacKinnon, Philip J. Johnson

Research output: Contribution to journal › Article › peer-review

Abstract

Glial cell line-derived neurotrophic factor (GDNF) has potent axonal growth and survival effects on motoneurons. This study used transgenic Myo-GDNF mice to assess the effects of targeted GDNF overexpression on functional recovery after botulinum toxin type A (BTxA) chemodenervation. BTxA (0.1 U) was injected into the tibialis anterior (TA) muscle of wild-type CF1 and transgenic Myo-GDNF mice. On days 1, 7, 14, and 21 after injection, evoked muscle force production and muscle mass were measured (n = 6, for each group at each time point). Greater maximal tetanic force and calculated specific force were evoked in Myo-GDNF animals when compared with control CF1 animals at days 1, 7, and 21. However, the differences were not statistically significant. Similarly, modest reductions in muscle atrophy in the Myo-GDNF group at all time points were not statistically significant. Targeted overexpression of GDNF in the muscles of Myo-GDNF mice did not improve motor recovery in the first 21 days after BTxA chemodenervation.

Original language	English (US)
Pages (from-to)	370-376
Number of pages	7
Journal	Microsurgery
Volume	32
Issue number	5
DOIs	https://doi.org/10.1002/micr.21967
State	Published - Jul 2012

ASJC Scopus subject areas

Surgery

Access to Document

10.1002/micr.21967

Cite this

@article{861e7f4763494823ab2e26d6c50f3d61,

title = "GDNF overexpression fails to provoke muscle recovery from botulinum toxin poisoning: A preliminary study",

abstract = "Glial cell line-derived neurotrophic factor (GDNF) has potent axonal growth and survival effects on motoneurons. This study used transgenic Myo-GDNF mice to assess the effects of targeted GDNF overexpression on functional recovery after botulinum toxin type A (BTxA) chemodenervation. BTxA (0.1 U) was injected into the tibialis anterior (TA) muscle of wild-type CF1 and transgenic Myo-GDNF mice. On days 1, 7, 14, and 21 after injection, evoked muscle force production and muscle mass were measured (n = 6, for each group at each time point). Greater maximal tetanic force and calculated specific force were evoked in Myo-GDNF animals when compared with control CF1 animals at days 1, 7, and 21. However, the differences were not statistically significant. Similarly, modest reductions in muscle atrophy in the Myo-GDNF group at all time points were not statistically significant. Targeted overexpression of GDNF in the muscles of Myo-GDNF mice did not improve motor recovery in the first 21 days after BTxA chemodenervation.",

author = "Lin, {Kenny F.} and Sun, {Hank H.} and MacEwan, {Matthew R.} and MacKinnon, {Susan E.} and Johnson, {Philip J.}",

year = "2012",

month = jul,

doi = "10.1002/micr.21967",

language = "English (US)",

volume = "32",

pages = "370--376",

journal = "Microsurgery",

issn = "0738-1085",

publisher = "Wiley",

number = "5",

}

TY - JOUR

T1 - GDNF overexpression fails to provoke muscle recovery from botulinum toxin poisoning

T2 - A preliminary study

AU - Lin, Kenny F.

AU - Sun, Hank H.

AU - MacEwan, Matthew R.

AU - MacKinnon, Susan E.

AU - Johnson, Philip J.

PY - 2012/7

Y1 - 2012/7

N2 - Glial cell line-derived neurotrophic factor (GDNF) has potent axonal growth and survival effects on motoneurons. This study used transgenic Myo-GDNF mice to assess the effects of targeted GDNF overexpression on functional recovery after botulinum toxin type A (BTxA) chemodenervation. BTxA (0.1 U) was injected into the tibialis anterior (TA) muscle of wild-type CF1 and transgenic Myo-GDNF mice. On days 1, 7, 14, and 21 after injection, evoked muscle force production and muscle mass were measured (n = 6, for each group at each time point). Greater maximal tetanic force and calculated specific force were evoked in Myo-GDNF animals when compared with control CF1 animals at days 1, 7, and 21. However, the differences were not statistically significant. Similarly, modest reductions in muscle atrophy in the Myo-GDNF group at all time points were not statistically significant. Targeted overexpression of GDNF in the muscles of Myo-GDNF mice did not improve motor recovery in the first 21 days after BTxA chemodenervation.

AB - Glial cell line-derived neurotrophic factor (GDNF) has potent axonal growth and survival effects on motoneurons. This study used transgenic Myo-GDNF mice to assess the effects of targeted GDNF overexpression on functional recovery after botulinum toxin type A (BTxA) chemodenervation. BTxA (0.1 U) was injected into the tibialis anterior (TA) muscle of wild-type CF1 and transgenic Myo-GDNF mice. On days 1, 7, 14, and 21 after injection, evoked muscle force production and muscle mass were measured (n = 6, for each group at each time point). Greater maximal tetanic force and calculated specific force were evoked in Myo-GDNF animals when compared with control CF1 animals at days 1, 7, and 21. However, the differences were not statistically significant. Similarly, modest reductions in muscle atrophy in the Myo-GDNF group at all time points were not statistically significant. Targeted overexpression of GDNF in the muscles of Myo-GDNF mice did not improve motor recovery in the first 21 days after BTxA chemodenervation.

UR - http://www.scopus.com/inward/record.url?scp=84863727560&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84863727560&partnerID=8YFLogxK

U2 - 10.1002/micr.21967

DO - 10.1002/micr.21967

M3 - Article

C2 - 22473739

AN - SCOPUS:84863727560

SN - 0738-1085

VL - 32

SP - 370

EP - 376

JO - Microsurgery

JF - Microsurgery

IS - 5

ER -

GDNF overexpression fails to provoke muscle recovery from botulinum toxin poisoning: A preliminary study

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this