[Gd@C82(OH)22]n nanoparticles induce dendritic cell maturation and activate Th1 immune responses

De Yang, Yuliang Zhao, Hua Guo, Yana Li, Poonam Tewary, Gengmei Xing, Wei Hou, Joost J. Oppenheim, Ning Zhang

Research output: Contribution to journalArticlepeer-review

109 Scopus citations

Abstract

Dendritic cells play a pivotal role in host immune defense, such as elimination of foreign pathogen and inhibition of tumorigenesis. In this paper, we report that [Gd@C82(OH)22]n could induce phenotypic maturation of dendritic cells by stimulating DC production of cytokines including IL-12p70, upregulating DC co-stimulatory (CD80, CD83, and CD86) and MHC (HLA-A,B,C and HLA-DR) molecules, and switching DCs from a CCL5-responsive to a CCL19-responsive phenotype. We found that [Gd@C 82(OH)22]n can induce dendritic cells to become functionally mature as illustrated by their capacity to activate allogeneic T cells. Mice immunized with ovalbumin in the presence of [Gd@C 82(OH)22]n exhibit enhanced ovalbumin-specific Th1-polarized immune response as evidenced by the predominantly increased production of IFN-, IL-1-, and IL-2. The [Gd@C82(OH) 22]n nanoparticle is a potent activator of dendritic cells and Th1 immune responses. These new findings also provide a rational understanding of the potent anticancer activities of [Gd@C82(OH) 22]n nanoparticles reported previously.

Original languageEnglish (US)
Pages (from-to)1178-1186
Number of pages9
JournalACS Nano
Volume4
Issue number2
DOIs
StatePublished - Feb 23 2010

Keywords

  • Activation
  • Dendritic cells
  • Fullerene
  • Nanomedicine
  • Th1 polarization

ASJC Scopus subject areas

  • Engineering(all)
  • Materials Science(all)
  • Physics and Astronomy(all)

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