Proof is presented that methyl esters of highly unsaturated long chain fatty acids are not significantly altered in chemical structure during gas-liquid chromatography with the stationary phase Apiezon M at 197°. Relative retention volumes of C16, C18, C20 and C22 polyenoic acids are listed for 2 stationary phases, non-polar Apiezon M and polar Reoplex 400. A system for rapid total analysis of complex fatty acid mixtures on a submilligram scale is described.
|Number of pages
|Proceedings of the Society for Experimental Biology and Medicine
|Published - Oct 1958
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)