Gas chromatography-mass spectrometry in analysis of protein amino acid composition

J. J. Rafter, M. Ingelman-Sundberg, Jan-Ake Gustafsson

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

A method for quantitative analysis of protein amino acids using isotope ratio gas chromatography-mass spectrometry computer techniques is described. The amino acids are analysed as their N-trifluoroacetyl-n-butyl esters and the quantitation is acccomplished by the inclusion of deuterated amino acid mixtures of known composition. The inclusion of internal standards prior to protein hydrolysis provides a method with high precision (coefficient of variation less than 5%) The method, which requires 100 μg and can be performed in just over 2 h, when tested on insulin gave results which agreed well with the known composition of the protein and with simultaneous analysis on ion exchangers. Thus, the technique offers an alternative to automatic amino acid analysers (utilizing ion exchangers) for quantitative amino acid analysis.

Original languageEnglish (US)
Pages (from-to)321-331
Number of pages11
JournalActa Biologica et Medica Germanica
Volume38
Issue number2-3
StatePublished - Dec 1 1979

ASJC Scopus subject areas

  • Medicine(all)

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