Ganglioside GM1 treatment prevents the effects of subacute exposure to toluene on N-[3H]propylnorapomorphine binding characteristics in rat striatal membranes

Gabriel von Euler; Kjell Fuxe; Luigi F. Agnati; Tiiu Hansson; Jan Åke Gustafsson

doi:10.1016/0304-3940(87)90126-1

Ganglioside GM₁ treatment prevents the effects of subacute exposure to toluene on N-[³H]propylnorapomorphine binding characteristics in rat striatal membranes

Gabriel von Euler, Kjell Fuxe, Luigi F. Agnati, Tiiu Hansson, Jan Åke Gustafsson

Houston Methodist

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

The effects of ganglioside GM₁ treatment (10 mg/kg, i.p., 3 days, once daily) were analyzed on N-[³H]propylnorapomorphine ([³H]NPA) binding characteristics in striatal membrane preparations from air or toluene (80 ppm, 3 days, 6 h/day) exposed adult male rats. It was found that toluene exposure induced increased K_d and B_max values for [³H]NPA binding. These changes could be blocked by GM₁ treatment, which by itself had no significant effects on the binding characteristics of [³H]NPA. The addition of toluene (9.3 μmol/ml) in vitro had similar effects on [³H]NPA binding characteristics as toluene exposure in vivo. These results indicate that the effects of toluene exposure on dopamine receptors are due to an alteration of membrane fluidity which is prevented by GM₁ treatment.

Original language	English (US)
Pages (from-to)	181-184
Number of pages	4
Journal	Neuroscience Letters
Volume	82
Issue number	2
DOIs	https://doi.org/10.1016/0304-3940(87)90126-1
State	Published - Nov 23 1987

Keywords

Dopamine receptor
Ganglioside GM
N-[H]Propylnorapomorphine
Rat
Striatum
Toluene

ASJC Scopus subject areas

General Neuroscience

Access to Document

10.1016/0304-3940(87)90126-1

Cite this

@article{ce040742ddfa41f1b9c9db721a9cf1ea,

title = "Ganglioside GM1 treatment prevents the effects of subacute exposure to toluene on N-[3H]propylnorapomorphine binding characteristics in rat striatal membranes",

abstract = "The effects of ganglioside GM1 treatment (10 mg/kg, i.p., 3 days, once daily) were analyzed on N-[3H]propylnorapomorphine ([3H]NPA) binding characteristics in striatal membrane preparations from air or toluene (80 ppm, 3 days, 6 h/day) exposed adult male rats. It was found that toluene exposure induced increased Kd and Bmax values for [3H]NPA binding. These changes could be blocked by GM1 treatment, which by itself had no significant effects on the binding characteristics of [3H]NPA. The addition of toluene (9.3 μmol/ml) in vitro had similar effects on [3H]NPA binding characteristics as toluene exposure in vivo. These results indicate that the effects of toluene exposure on dopamine receptors are due to an alteration of membrane fluidity which is prevented by GM1 treatment.",

keywords = "Dopamine receptor, Ganglioside GM, N-[H]Propylnorapomorphine, Rat, Striatum, Toluene",

author = "{von Euler}, Gabriel and Kjell Fuxe and Agnati, {Luigi F.} and Tiiu Hansson and Gustafsson, {Jan {\AA}ke}",

note = "Funding Information: This work has been supportedb y Grant 84-1300f rom the Swedish Work Environment Fund, Stockholm, Sweden. We are grateful to Ulla-Britt Finnman and Birgitta Nyberg for outstandingt echnicaal ssistance. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1987",

month = nov,

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doi = "10.1016/0304-3940(87)90126-1",

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T1 - Ganglioside GM1 treatment prevents the effects of subacute exposure to toluene on N-[3H]propylnorapomorphine binding characteristics in rat striatal membranes

AU - von Euler, Gabriel

AU - Fuxe, Kjell

AU - Agnati, Luigi F.

AU - Hansson, Tiiu

AU - Gustafsson, Jan Åke

N1 - Funding Information: This work has been supportedb y Grant 84-1300f rom the Swedish Work Environment Fund, Stockholm, Sweden. We are grateful to Ulla-Britt Finnman and Birgitta Nyberg for outstandingt echnicaal ssistance. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1987/11/23

Y1 - 1987/11/23

N2 - The effects of ganglioside GM1 treatment (10 mg/kg, i.p., 3 days, once daily) were analyzed on N-[3H]propylnorapomorphine ([3H]NPA) binding characteristics in striatal membrane preparations from air or toluene (80 ppm, 3 days, 6 h/day) exposed adult male rats. It was found that toluene exposure induced increased Kd and Bmax values for [3H]NPA binding. These changes could be blocked by GM1 treatment, which by itself had no significant effects on the binding characteristics of [3H]NPA. The addition of toluene (9.3 μmol/ml) in vitro had similar effects on [3H]NPA binding characteristics as toluene exposure in vivo. These results indicate that the effects of toluene exposure on dopamine receptors are due to an alteration of membrane fluidity which is prevented by GM1 treatment.

AB - The effects of ganglioside GM1 treatment (10 mg/kg, i.p., 3 days, once daily) were analyzed on N-[3H]propylnorapomorphine ([3H]NPA) binding characteristics in striatal membrane preparations from air or toluene (80 ppm, 3 days, 6 h/day) exposed adult male rats. It was found that toluene exposure induced increased Kd and Bmax values for [3H]NPA binding. These changes could be blocked by GM1 treatment, which by itself had no significant effects on the binding characteristics of [3H]NPA. The addition of toluene (9.3 μmol/ml) in vitro had similar effects on [3H]NPA binding characteristics as toluene exposure in vivo. These results indicate that the effects of toluene exposure on dopamine receptors are due to an alteration of membrane fluidity which is prevented by GM1 treatment.

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KW - Striatum

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