opotentiates estrogen receptor α activity via the ERK signaling pathway

Melyssa R. Bratton, James WAntoon, Bich N. Duong, Daniel E. Frigo, Syreeta Tilghman, Bridgette M. Collins-Burow, Steven Elliott, Yan Tang, Lilia I. Melnik, Ling Lai, Jawed Alam, Barbara S. Beckman, Steven M. Hill, Brian G. Rowan, John A. McLachlan, Matthew E. Burow

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

The estrogen receptor α (ERα) is a transcription factor that mediates the biological effects of 17β-estradiol (E2). ERa transcriptional activity is also regulated by cytoplasmic signaling cascades. Here, several Ga protein subunits were tested for their ability to regulate ERα activity. Reporter assays revealed that overexpression of a constitutively active Gα protein subunit potentiated ERα activity in the absence and presence of E2. Transient transfection of the human breast cancer cell line MCF-7 showed that Gαo augments the transcription of several ERα-regulated genes. Western blots of HEK293T cells transfected with ERGGao revealed that Gao stimulated phosphorylation of ERK 1/2 and subsequently increased the phosphorylation of ERα on serine 118. In summary, our results show that Gαo, through activation of the MAPK pathway, plays a role in the regulation of ERα activity.

Original languageEnglish (US)
Pages (from-to)45-54
Number of pages10
JournalJournal of Endocrinology
Volume214
Issue number1
DOIs
StatePublished - Jul 2012

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

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