Functionalized viral nanoparticles as ultrasensitive reporters in lateral-flow assays

Meena Adhikari; Sagar Dhamane; Anna E.V. Hagström; Gavin Garvey; Wen Hsiang Chen; Katerina Kourentzi; Ulrich Strych; Richard C. Willson

doi:10.1039/c3an00891f

Functionalized viral nanoparticles as ultrasensitive reporters in lateral-flow assays

Meena Adhikari, Sagar Dhamane, Anna E.V. Hagström, Gavin Garvey, Wen Hsiang Chen, Katerina Kourentzi, Ulrich Strych, Richard C. Willson

Houston Methodist

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

Two types of viral nanoparticles were functionalized with target-specific antibodies and multiple copies of an enzymatic reporter (horseradish peroxidase). The particles were successfully integrated into an immunochromatographic assay detecting MS2 bacteriophage, a model for viral pathogens. The sensitivity of the assay was greatly superior to conventional gold nanoparticle lateral flow assays, and results could easily be evaluated, even without advanced lab instruments. This journal is

Original language	English (US)
Pages (from-to)	5584-5587
Number of pages	4
Journal	Analyst
Volume	138
Issue number	19
DOIs	https://doi.org/10.1039/c3an00891f
State	Published - Oct 7 2013

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Environmental Chemistry
Spectroscopy
Electrochemistry

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AU - Kourentzi, Katerina

AU - Strych, Ulrich

AU - Willson, Richard C.

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Functionalized viral nanoparticles as ultrasensitive reporters in lateral-flow assays

Abstract

ASJC Scopus subject areas

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