Functional Properties and Effect on Growth Suppression of Human Neuroblastoma Tumors by Isotype Switch Variants of Monoclonal Antiganglioside Gd2 Antibody 14.18

Kalpana Mujoo, Hsin Ming Yang, David A. Cheresh, Ursula Wargalla, Diana J. Sander, Ralph A. Reisfeld, Thomas J. Kipps

Research output: Contribution to journalArticle

141 Scopus citations

Abstract

A complete family of IgG isotype switch variant hybridomas was generated from the anti-Gd2 monoclonal IgG3-prodncing hybridoma, 14.18, with the aid of the fluorescence-activated cell sorter. The IgGl, IgG2b, and IgG2a monoclonal antibodies (Mabs) produced by respective isotype switch variant hybridomas 14G1,14G2b, or 14G2a, have binding activities for the biochemically defined GD2 antigen and GD2-expressing neuroblastoma target cell lines identical to that of IgG3 Mabs produced by the 14.18 parent cell line. This permitted us to examine the relative in vitro and in vivo cytotoxic capacities of each of the anti-GD2 antibodies for GD2-expressing neuroblastoma cells independent of antibody binding affinity or specificity. Mabs produced by 14.18,14G2a, or 14G2b, but not 14G1, can direct efficient complement-dependent cytotoxicity against neuroblastoma tumor cells in the presence of human complement. Mabs produced by the parent 14.18 or by 14G2a are more efficient in directing antibody-dependent cell-mediated cytotoxicity than Mabs produced by 14G2b, and Mabs of 14G1 are inactive. However, despite these noted in vitro differences, antibodies produced by each member of this switch variant family suppress the growth of human neuroblastoma tumor cells in BALB/c athymic nu/nu mice. These studies suggest that a mechanism(s) other than Fc-directed complement-dependent cytotoxicity or antibody-dependent cell-mediated cytotoxicity may account for the in vivo antitumor effects of these particular antibodies.

Original languageEnglish (US)
Pages (from-to)2857-2861
Number of pages5
JournalCancer research
Volume49
Issue number11
StatePublished - Jun 1 1989

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Functional Properties and Effect on Growth Suppression of Human Neuroblastoma Tumors by Isotype Switch Variants of Monoclonal Antiganglioside Gd2 Antibody 14.18'. Together they form a unique fingerprint.

Cite this