Functional Immune Cell-Derived Exosomes Engineered for the Trilogy of Radiotherapy Sensitization

Xiaotu Ma, Meinan Yao, Yu Gao, Yale Yue, Yao Li, Tianjiao Zhang, Guangjun Nie, Xiao Zhao, Xiaolong Liang

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


The limited efficacy of radiotherapy leads to radio-resistance and high rates of tumor recurrence and metastasis, which is caused by tumor hypoxia, rapid DNA damage repair, and especially the suppressive immune microenvironment of tumor. Lots of immune cell-derived exosomes can regulate antitumor immunity, but their application in enhancing radiotherapy is rarely studied. Herein, as a model of concept, M1 macrophage-derived exosomes (M1Exos) is engineered as effective radiotherapy sensitizers, realizing the trilogy of radiotherapy sensitization: 1) M1Exos is engineered to express catalases on the inside of membrane, which can effectively relieve tumor hypoxia, and enhance DNA damage. 2) The DNA damage repair inhibitor is loaded in M1Exos to effectively inhibit DNA damage repair. 3) M1Exos can polarize M2 macrophages into M1 phenotypes, and the anti-PD-L1 nanobody engineered on the outside of M1Exos can relieve the immunosuppression of T cells, both ultimately leading to the remodeling of the tumor suppressive microenvironment. The trilogy of radiotherapy sensitization achieves excellent antitumor efficacy, exhibiting the good utility of engineering immune cell-derived exosomes as radiotherapy sensitizers, inspiring the future efforts to explore different kinds of immune cell-derived exosomes for enhanced radiotherapy.

Original languageEnglish (US)
Article number2106031
JournalAdvanced Science
Issue number23
StatePublished - Aug 15 2022


  • engineered exosome
  • immune microenvironment
  • macrophage polarization
  • radiotherapy sensitization
  • tumor hypoxia

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Chemical Engineering(all)
  • Materials Science(all)
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)
  • Engineering(all)
  • Physics and Astronomy(all)


Dive into the research topics of 'Functional Immune Cell-Derived Exosomes Engineered for the Trilogy of Radiotherapy Sensitization'. Together they form a unique fingerprint.

Cite this