TY - JOUR
T1 - Functional characterization of α1-adrenergic receptors in experimental vein grafts
AU - Davies, Mark G.
AU - Huynh, Tam T.T.
AU - Hagen, Per Otto
N1 - Funding Information:
1This work was supported by U.S. Public Health Service Grant HL-15448. Mark G. Davies is supported by a National Institutes of Health Fogarty International Research Fellowship (TW 04810). 2To whom correspondence should be addressed at Duke University Medical Center, P.O. Box 3473, Durham, NC 27710. Fax: (919) 684–5293. E-mail: [email protected].
PY - 1999/6/1
Y1 - 1999/6/1
N2 - Background. Studies on the pharmacology of the smooth muscle cells in vein bypass grafts suggest that the function of G-proteins and adrenergic receptors is altered. This study examines the α-adrenergic responsiveness of smooth muscle cells in vein bypass grafts as compared with those in the common carotid arteries and external jugular veins. Methods. New Zealand White rabbits received jugular vein interposition bypass grafts of the common carotid. Vessel segments of the vein bypass grafts harvested after 28 days, common carotid arteries, and external jugular veins were sectioned into 5-mm rings (four per vessel) for studies of isometric tension in response to phenylephrine (10-10 to 10-4 M) alone and in the presence of prazosin, an α-adrenergic antagonist; WB4101 and 5-methylurapidil (5-MU), α(1A), antagonists; chloroethylclonidine (CEC); an α(1B) antagonist; or the G(i/o) G-protein inhibitor pertussis toxin (PTx). Results. All vessels had prazosin- sensitive responses. The jugular veins appear to have functional α(1A), receptors (WB4101 and 5-MU sensitive, CEC insensitive) which are associated with pertussis toxin-sensitive G-proteins. Carotid arteries appear to have atypical α1, receptors (WB4101 and 5-MU insensitive, CEC insensitive) associated with pertussis toxininsensitive G-proteins. Vein grafts appear to have functional α(1B) receptors (WB4101 and 5-MU insensitive, CEC sensitive) which are associated with pertussis toxin-insensitive G-proteins. Conclusions. These results show that placement of a vein into the arterial circulation induces a change in α1-adrenergic receptor subtypes (α(1A) to α(1B)) and in the G-protein coupling of the receptors (PTx sensitive to PTx insensitive), reflecting a significant phenotypic change in smooth muscle cell signal transduction.
AB - Background. Studies on the pharmacology of the smooth muscle cells in vein bypass grafts suggest that the function of G-proteins and adrenergic receptors is altered. This study examines the α-adrenergic responsiveness of smooth muscle cells in vein bypass grafts as compared with those in the common carotid arteries and external jugular veins. Methods. New Zealand White rabbits received jugular vein interposition bypass grafts of the common carotid. Vessel segments of the vein bypass grafts harvested after 28 days, common carotid arteries, and external jugular veins were sectioned into 5-mm rings (four per vessel) for studies of isometric tension in response to phenylephrine (10-10 to 10-4 M) alone and in the presence of prazosin, an α-adrenergic antagonist; WB4101 and 5-methylurapidil (5-MU), α(1A), antagonists; chloroethylclonidine (CEC); an α(1B) antagonist; or the G(i/o) G-protein inhibitor pertussis toxin (PTx). Results. All vessels had prazosin- sensitive responses. The jugular veins appear to have functional α(1A), receptors (WB4101 and 5-MU sensitive, CEC insensitive) which are associated with pertussis toxin-sensitive G-proteins. Carotid arteries appear to have atypical α1, receptors (WB4101 and 5-MU insensitive, CEC insensitive) associated with pertussis toxininsensitive G-proteins. Vein grafts appear to have functional α(1B) receptors (WB4101 and 5-MU insensitive, CEC sensitive) which are associated with pertussis toxin-insensitive G-proteins. Conclusions. These results show that placement of a vein into the arterial circulation induces a change in α1-adrenergic receptor subtypes (α(1A) to α(1B)) and in the G-protein coupling of the receptors (PTx sensitive to PTx insensitive), reflecting a significant phenotypic change in smooth muscle cell signal transduction.
KW - α adrenoceptor
KW - Function
KW - Intimal hyperplasia
KW - Rabbit
KW - Smooth muscle cells
KW - Vein grafts
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U2 - 10.1006/jsre.1999.5600
DO - 10.1006/jsre.1999.5600
M3 - Article
C2 - 10334887
AN - SCOPUS:0033137353
SN - 0022-4804
VL - 84
SP - 40
EP - 45
JO - Journal of Surgical Research
JF - Journal of Surgical Research
IS - 1
ER -