Frequent inactivation of p16(INK4α) in oral premalignant lesions

Vali Papadimitrakopoulou, Julie Izzo, Scott M. Lippman, Jin Soo Lee, You Hong Fan, Gary Clayman, Jay Y. Ro, Walter N. Hittelman, Reuben Lotan, Waun K. Hong, Li Mao

Research output: Contribution to journalArticlepeer-review

156 Scopus citations


Head and neck carcinogenesis is believed to be a multistep process, whereby genetic events accumulate in the carcinogen-exposed field at risk, resulting in distinct phenotypic premalignant changes that eventually evolve into invasive cancer. Frequent loss of heterozygosity (LOH) at the chromosome 9p21 region and inactivation of p16(INK4α) by different mechanisms have been described in head and neck squamous cell carcinoma (HNSCC). Recently, we reported that loss of 9p21 is also frequent in oral premalignant lesions. To investigate potential inactivation of p16(INK4α) in these premalignant lesions, we analysed 74 biopsies from 36 patients by immunohistochemistry (IHC) for expression of the p16 protein. Loss of p16 expression was found in 28 (38%) of the lesion biopsies from 17 patients (47%). LOH at the D9s171, a marker in the 9p21 region, was observed in 19 lesion biopsies from 12 cases and correlated with absence of p16 by IHC in 11 (92%) of the 12 comparable cases and 15 (79%) of 19 lesion biopsies. By direct sequencing of ten lesion biopsies from ten individuals with LOH at D9s171 for p16(INK4α) exon 2, one non-sense mutation at codon 88 (GGA→TGA) was identified. Our data suggest that inactivation of p16(INK4α) may play an important role in early head and neck cancer development.

Original languageEnglish (US)
Pages (from-to)1799-1803
Number of pages5
Issue number15
StatePublished - 1997


  • Head and neck cancer
  • Immunohistochemistry
  • Oral premalignant lesions
  • Pl6(INK4α)

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology


Dive into the research topics of 'Frequent inactivation of p16(INK4α) in oral premalignant lesions'. Together they form a unique fingerprint.

Cite this