The widely distributed bacterial σ 54-dependent transcription regulates pathogenicity and numerous adaptive responses in diverse bacteria. Formation of the σ 54-dependent open promoter complex is a multi-step process driven by AAA + ATPases. Non-hydrolysable nucleotide analogues are particularly suitable for studying such complexity by capturing various intermediate states along the energy coupling pathway. Here we report a novel ATP analogue, ADP-MgF 3 -, which traps an AAA + ATPase with its target σ 54. The MgF 3 --dependent complex is highly homogeneous and functional assays suggest it may represent an early transcription intermediate state valuable for structural studies.
- AAA ATPase
- Nucleotide analogue
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)