Formation of MgF 3 --dependent complexes between an AAA + ATPase and σ 54

Nan Zhang, Martin Buck

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

The widely distributed bacterial σ 54-dependent transcription regulates pathogenicity and numerous adaptive responses in diverse bacteria. Formation of the σ 54-dependent open promoter complex is a multi-step process driven by AAA + ATPases. Non-hydrolysable nucleotide analogues are particularly suitable for studying such complexity by capturing various intermediate states along the energy coupling pathway. Here we report a novel ATP analogue, ADP-MgF 3 -, which traps an AAA + ATPase with its target σ 54. The MgF 3 --dependent complex is highly homogeneous and functional assays suggest it may represent an early transcription intermediate state valuable for structural studies.

Original languageEnglish (US)
Pages (from-to)89-92
Number of pages4
JournalFEBS Open Bio
Volume2
DOIs
StatePublished - 2012

Keywords

  • AAA ATPase
  • BEBP
  • Nucleotide analogue
  • PspF
  • Transcription
  • σ

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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