Folate-conjugated liposomes preferentially target macrophages associated with ovarian carcinoma

Mary Jo Turk; David J. Waters; Philip S. Low

doi:10.1016/j.canlet.2003.12.028

Folate-conjugated liposomes preferentially target macrophages associated with ovarian carcinoma

Mary Jo Turk, David J. Waters, Philip S. Low

Houston Methodist

Research output: Contribution to journal › Article › peer-review

178 Scopus citations

Abstract

The folate receptor (FR) is overexpressed on many epithelial cancers and has been exploited for targeted delivery of folate-linked liposomes to cancer cells in vitro. The present studies investigate the distribution of folate-targeted liposomes in a FR(+) mouse model of ovarian cancer. According to flow cytometric analysis, folate-conjugation of liposomes significantly enhanced their uptake into ovarian cancer cells and tumor-associated macrophages within tumor ascites fluid. Compared to ovarian cancer cells, macrophages acquired tenfold more liposomes, and approximately 50% of this uptake was FR-dependent. These results demonstrate that, in addition to their cancer cell-targeting properties, folate-liposomes may be useful for targeting drugs to tumor-associated macrophages in vivo.

Original language	English (US)
Pages (from-to)	165-172
Number of pages	8
Journal	Cancer Letters
Volume	213
Issue number	2
DOIs	https://doi.org/10.1016/j.canlet.2003.12.028
State	Published - Sep 30 2004

Keywords

Folate receptor targeting
Liposomal drug delivery
Ovarian cancer therapy
Tumor macrophages

ASJC Scopus subject areas

Cancer Research
Molecular Biology
Oncology

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10.1016/j.canlet.2003.12.028

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title = "Folate-conjugated liposomes preferentially target macrophages associated with ovarian carcinoma",

abstract = "The folate receptor (FR) is overexpressed on many epithelial cancers and has been exploited for targeted delivery of folate-linked liposomes to cancer cells in vitro. The present studies investigate the distribution of folate-targeted liposomes in a FR(+) mouse model of ovarian cancer. According to flow cytometric analysis, folate-conjugation of liposomes significantly enhanced their uptake into ovarian cancer cells and tumor-associated macrophages within tumor ascites fluid. Compared to ovarian cancer cells, macrophages acquired tenfold more liposomes, and approximately 50% of this uptake was FR-dependent. These results demonstrate that, in addition to their cancer cell-targeting properties, folate-liposomes may be useful for targeting drugs to tumor-associated macrophages in vivo.",

keywords = "Folate receptor targeting, Liposomal drug delivery, Ovarian cancer therapy, Tumor macrophages",

author = "Turk, {Mary Jo} and Waters, {David J.} and Low, {Philip S.}",

note = "Funding Information: The authors would like to thank Kathy Ragheb for her assistance with flow cytometry, and Maxine Nichols for inoculation of mice. This research was supported by grants from Endocyte, Inc. (West Lafayette, IN) and the National Institutes of Health (CA 89581 and GM 08298).",

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T1 - Folate-conjugated liposomes preferentially target macrophages associated with ovarian carcinoma

AU - Turk, Mary Jo

AU - Waters, David J.

AU - Low, Philip S.

N1 - Funding Information: The authors would like to thank Kathy Ragheb for her assistance with flow cytometry, and Maxine Nichols for inoculation of mice. This research was supported by grants from Endocyte, Inc. (West Lafayette, IN) and the National Institutes of Health (CA 89581 and GM 08298).

PY - 2004/9/30

Y1 - 2004/9/30

N2 - The folate receptor (FR) is overexpressed on many epithelial cancers and has been exploited for targeted delivery of folate-linked liposomes to cancer cells in vitro. The present studies investigate the distribution of folate-targeted liposomes in a FR(+) mouse model of ovarian cancer. According to flow cytometric analysis, folate-conjugation of liposomes significantly enhanced their uptake into ovarian cancer cells and tumor-associated macrophages within tumor ascites fluid. Compared to ovarian cancer cells, macrophages acquired tenfold more liposomes, and approximately 50% of this uptake was FR-dependent. These results demonstrate that, in addition to their cancer cell-targeting properties, folate-liposomes may be useful for targeting drugs to tumor-associated macrophages in vivo.

AB - The folate receptor (FR) is overexpressed on many epithelial cancers and has been exploited for targeted delivery of folate-linked liposomes to cancer cells in vitro. The present studies investigate the distribution of folate-targeted liposomes in a FR(+) mouse model of ovarian cancer. According to flow cytometric analysis, folate-conjugation of liposomes significantly enhanced their uptake into ovarian cancer cells and tumor-associated macrophages within tumor ascites fluid. Compared to ovarian cancer cells, macrophages acquired tenfold more liposomes, and approximately 50% of this uptake was FR-dependent. These results demonstrate that, in addition to their cancer cell-targeting properties, folate-liposomes may be useful for targeting drugs to tumor-associated macrophages in vivo.

KW - Folate receptor targeting

KW - Liposomal drug delivery

KW - Ovarian cancer therapy

KW - Tumor macrophages

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Folate-conjugated liposomes preferentially target macrophages associated with ovarian carcinoma

Abstract

Keywords

ASJC Scopus subject areas

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