FMS-like tyrosine kinase 3 interacts with the glucocorticoid receptor complex and affects glucocorticoid dependent signaling

Abolfazl Asadi, Erik Hedman, Christina Widén, Johanna Zilliacus, Jan Åke Gustafsson, Ann Charlotte Wikström

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

The glucocorticoid receptor (GR) forms part of a multiprotein complex consisting of chaperones and proteins active in glucocorticoid signaling and other pathways. By immunoaffinity purification of GR, followed by Edman sequencing and Western blotting, we identified the FMS-like tyrosine kinase 3 (Flt3) as a GR-interacting protein in rat liver and hepatoma cells. Flt3 interacts with both non-liganded and liganded GR. The DNA-binding domain of GR is sufficient for Flt3 interaction as shown by GST-pull down experiments. Studies of the effects of Flt3 and its ligand FL in glucocorticoid-driven reporter-gene assays in Cos7 cells, show that co-transfection with Flt3 and FL potentiates glucocorticoid effects. Treatment with FL had no effect on GR location and Dex induced translocation of GR was unaffected by FL. In summary, GR and Flt3 interact, affecting GR signaling. This novel cross-talk between GR and a hematopoietic growth factor might also imply glucocorticoid effects on Flt3-mediated signaling.

Original languageEnglish (US)
Pages (from-to)569-574
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume368
Issue number3
DOIs
StatePublished - Apr 11 2008

Keywords

  • Flt3
  • Glucocorticoid receptor
  • Glucocorticoids
  • GST-pull down
  • Immunoprecipitation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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