Fluorescent QDs-polystyrene composite nanospheres for highly efficient and rapid protein antigen detection

Changhua Zhou, Mao Mao, Hang Yuan, Huaibin Shen, Feng Wu, Lan Ma, Lin Song Li

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


In this paper, high-quality carboxyl-functionalized fluorescent (red, green, and blue emitting) nanospheres (46-103 nm) consisting of hydrophobic quantum dots (QDs) and polystyrene were prepared by a miniemulsion polymerization approach. This miniemulsion polymerization approach induced a homogeneous distribution and high aqueous-phase transport efficiency of fluorescent QDs in composite nanospheres, which proved the success of our encoding QDs strategy. The obtained fluorescent nanospheres exhibited high stability in aqueous solution under a wide range of pH, different salt concentrations, PBS buffer, and thermal treatment at 80°C. Based on the red emitting composite nanosphere, we performed fluorescent lateral flow immunoassay (LFIA) strips for high-sensitivity and rapid alpha-fetal protein detection. The detection limit reached 0.1 ng/mL, which was 200 times higher than commercial colloidal gold-labeled LFIA strips, and it reached similar detection level in enzyme-linked immunosorbent assay kit.

Original languageEnglish (US)
Article number1901
JournalJournal of Nanoparticle Research
Issue number9
StatePublished - Sep 2013


  • Composite nanospheres
  • Fluorescent QDs
  • Miniemulsion polymerization
  • Polystyrene

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Condensed Matter Physics
  • Modeling and Simulation
  • Chemistry(all)
  • Materials Science(all)
  • Bioengineering


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