Fluorescent primer-based in vitro transcription system of viral RNA-dependent RNA polymerases

Qiang Wang, Leiyun Weng, Hongbing Jiang, Shijian Zhang, Tetsuya Toyoda

Research output: Contribution to journalArticle

Abstract

Viral infection is a leading cause of disease and death. Although vaccines are the most effective method of controlling viral infections, antiviral drugs are also important. Here, we established an in vitro transcription system by using fluorescein isothiocyanate-conjugated primers for RNA polymerases of viruses that are important disease-causing human pathogens (influenza, hepatitis C, Japanese encephalitis viruses, and enterovirus 71). This technology will allow us to analyze RNA polymerase activity without using radioisotopes.

Original languageEnglish (US)
Pages (from-to)92-94
Number of pages3
JournalAnalytical Biochemistry
Volume433
Issue number2
DOIs
StatePublished - Feb 15 2013

Keywords

  • Enterovirus 71
  • Fluorescein isothiocyanate
  • Hepatitis C virus
  • In vitro transcription
  • Influenza virus
  • Japanese encephalitis virus

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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