TY - JOUR
T1 - Fluorescent cholesteryl esters in the core of low density lipoprotein
AU - Sklar, Larry A.
AU - Mantulin, William W.
AU - Pownall, Henry J.
N1 - Funding Information:
work was supported in part by National HL 19459 and HL 17269, and the American L.A.S. was supported in part by a Helen fellowship and National Institutes received support in part from the Texas Affiliate.
PY - 1982/3/30
Y1 - 1982/3/30
N2 - Fluorescent cholesteryl esters with chromophores placed either on the acyl chain (cholesteryl-cis-parinarate), the sterol ring system (5,7,9-cholesteryl oleate), or the side chain (naphthylcholenamide oleate) have been incorporated into the core of low density lipoprotein. The temperature dependence of several fluorescence parameters has been evaluated. An analysis of the fluorescence lifetime components of cholesteryl-cis-parinarate reveals coexisting environments whose proportion varies and reflects the thermotropic reorganization of the core of the particle. An analysis of the motion by dynamic depolarization suggests that the motions of the acyl chains in the core of the particle are highly restricted.
AB - Fluorescent cholesteryl esters with chromophores placed either on the acyl chain (cholesteryl-cis-parinarate), the sterol ring system (5,7,9-cholesteryl oleate), or the side chain (naphthylcholenamide oleate) have been incorporated into the core of low density lipoprotein. The temperature dependence of several fluorescence parameters has been evaluated. An analysis of the fluorescence lifetime components of cholesteryl-cis-parinarate reveals coexisting environments whose proportion varies and reflects the thermotropic reorganization of the core of the particle. An analysis of the motion by dynamic depolarization suggests that the motions of the acyl chains in the core of the particle are highly restricted.
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U2 - 10.1016/0006-291X(82)91487-5
DO - 10.1016/0006-291X(82)91487-5
M3 - Article
C2 - 7092879
AN - SCOPUS:0020493611
SN - 0006-291X
VL - 105
SP - 674
EP - 680
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -