Flow-induced immobilization of glucose oxidase in nonionic micellar nanogels for glucose sensing

Joshua J. Cardiel; Ya Zhao; Lige Tonggu; Liguo Wang; Jae Hyun Chung; Amy Q. Shen

doi:10.1039/c4lc00610k

Flow-induced immobilization of glucose oxidase in nonionic micellar nanogels for glucose sensing

Joshua J. Cardiel, Ya Zhao, Lige Tonggu, Liguo Wang, Jae Hyun Chung, Amy Q. Shen

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

A simple microfluidic platform was utilized to immobilize glucose oxidase (GOx) in a nonionic micellar scaffold. The immobilization of GOx was verified by using a combination of cryogenic electron microscopy (cryo-EM), scanning electron microscopy (SEM), and ultraviolet spectroscopy (UV) techniques. Chronoamperometric measurements were conducted on nanogel-GOx scaffolds under different glucose concentrations, exhibiting linear amperometric responses. Without impacting the lifetime and denaturation of GOx, the nonionic nanogel provides a favorable microenvironment for GOx in biological media. This flow-induced immobilization method in a nonionic nanogel host matrix opens up new pathways for designing a simple, fast, biocompatible, and cost-effective process to immobilize biomolecules that are averse to ionic environments.

Original language	English (US)
Pages (from-to)	3912-3916
Number of pages	5
Journal	Lab on a Chip
Volume	14
Issue number	20
DOIs	https://doi.org/10.1039/c4lc00610k
State	Published - Oct 21 2014

ASJC Scopus subject areas

Bioengineering
Biochemistry
General Chemistry
Biomedical Engineering

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title = "Flow-induced immobilization of glucose oxidase in nonionic micellar nanogels for glucose sensing",

abstract = "A simple microfluidic platform was utilized to immobilize glucose oxidase (GOx) in a nonionic micellar scaffold. The immobilization of GOx was verified by using a combination of cryogenic electron microscopy (cryo-EM), scanning electron microscopy (SEM), and ultraviolet spectroscopy (UV) techniques. Chronoamperometric measurements were conducted on nanogel-GOx scaffolds under different glucose concentrations, exhibiting linear amperometric responses. Without impacting the lifetime and denaturation of GOx, the nonionic nanogel provides a favorable microenvironment for GOx in biological media. This flow-induced immobilization method in a nonionic nanogel host matrix opens up new pathways for designing a simple, fast, biocompatible, and cost-effective process to immobilize biomolecules that are averse to ionic environments.",

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AU - Cardiel, Joshua J.

AU - Zhao, Ya

AU - Tonggu, Lige

AU - Wang, Liguo

AU - Chung, Jae Hyun

AU - Shen, Amy Q.

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Flow-induced immobilization of glucose oxidase in nonionic micellar nanogels for glucose sensing

Abstract

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