Flow-induced immobilization of glucose oxidase in nonionic micellar nanogels for glucose sensing

Joshua J. Cardiel, Ya Zhao, Lige Tonggu, Liguo Wang, Jae Hyun Chung, Amy Q. Shen

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

A simple microfluidic platform was utilized to immobilize glucose oxidase (GOx) in a nonionic micellar scaffold. The immobilization of GOx was verified by using a combination of cryogenic electron microscopy (cryo-EM), scanning electron microscopy (SEM), and ultraviolet spectroscopy (UV) techniques. Chronoamperometric measurements were conducted on nanogel-GOx scaffolds under different glucose concentrations, exhibiting linear amperometric responses. Without impacting the lifetime and denaturation of GOx, the nonionic nanogel provides a favorable microenvironment for GOx in biological media. This flow-induced immobilization method in a nonionic nanogel host matrix opens up new pathways for designing a simple, fast, biocompatible, and cost-effective process to immobilize biomolecules that are averse to ionic environments.

Original languageEnglish (US)
Pages (from-to)3912-3916
Number of pages5
JournalLab on a Chip
Volume14
Issue number20
DOIs
StatePublished - Oct 21 2014

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Fingerprint Dive into the research topics of 'Flow-induced immobilization of glucose oxidase in nonionic micellar nanogels for glucose sensing'. Together they form a unique fingerprint.

Cite this