Flow cytometric immunophenotyping in fine-needle aspiration of lymph nodes

Jaegul Chung, Gyungyub Gong, Jooryung Huh, Shin Kwang Khang, Jae Y. Ro

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Fine-needle aspiration (FNA) of lymph nodes has been regarded as a useful method in the diagnosis of lymphadenopathy. However, this procedure has been shown to be of limited value in the diagnosis of low or intermediate grade malignant lymphomas in some studies. Immunophenotyping is an essential adjunct to cytomorphology for the diagnosis of lymphoma by FNA. Immunophenotyping using flow cytometry (FCM) is rapid, objective and reliable. Using FCM, multiparametric analysis of 33 FNA materials from lymph nodes was performed and profiles of surface markers of lymphoid cells were assessed. In reactive hyperplasia, patterns of cell surface markers were quite variable, but disclosed polydonality. Most of the B-cell lymphomas showed immunophenotypes for B-cell lineages with their κ:λ or λ:κ ratio being over 3:1. In T-cell lymphomas, T-cell surface markers were predominantly expressed as well. In conclusion, our results suggest that immunophenotyping of lymph node aspirates is a valuable diagnostic adjunct for lymphoproliferative disorders, particularly in B-cell lymphomas because immunophenotyping can be easily and adequately performed by FCM.

Original languageEnglish (US)
Pages (from-to)393-400
Number of pages8
JournalJournal of Korean Medical Science
Volume14
Issue number4
DOIs
StatePublished - Aug 1999

Keywords

  • Flow cytometiy
  • Immunophenotyping
  • Lymphoproliferative disorders
  • Needle biopsy

ASJC Scopus subject areas

  • Medicine(all)

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