Fibronectin binding protein A of Staphylococcus aureus can mediate human T lymphocyte adhesion and coactivation

Y. J. Miyamoto, E. R. Wann, T. Fowler, E. Duffield, M. Höök, B. W. McIntyre

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

The extracellular matrix protein fibronectin (FN) mediates the adhesion of bacteria as well as T lymphocytes. Mammalian cells express integrins α4β1 and α5β1 as the major FN-binding cell surface receptors. Bacteria such as Staphylococcus aureus, also express FN-binding receptors that are important for adherence to host tissue and initiation of infection. The S. aureus FN-binding protein, FnbpA, has been previously identified, and recombinant proteins that correspond to distinct functional regions of this protein have been made. Three recombinant truncated forms of FnbpA, rFnbpA(37-881), rFnbpA(37-605), and rFnbpA(620-881), were examined for effects on in vitro adhesion and coactivation of human T lymphocytes. These proteins, when coimmobilized with anti-CD3 mAb, activated T lymphocyte proliferation. The coactivation signal generated by the rFnbpA proteins required medium containing serum with FN. Furthermore, the costimulatory signal could be restored in FN-depleted serum when the rFnbpAs were preloaded with soluble FN. Monoclonal Ab blocking studies revealed that integrin α5β1 is the major receptor responsible for the rFnbpA costimulatory signal. Shear flow cell detachment assays confirmed that lymphocytes can bind to FN captured by the rFnbpA proteins. These results suggest that the S. aureus rFnbpA can interact with integrin α5β1 via an FN bridge to mediate adhesion and costimulatory signals to T lymphocytes.

Original languageEnglish (US)
Pages (from-to)5129-5138
Number of pages10
JournalJournal of Immunology
Volume166
Issue number8
DOIs
StatePublished - Apr 15 2001

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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