TY - JOUR
T1 - Feminization of hepatic S metabolism in male rats with a transplanted (MtT/F4)
AU - Eneroth, Peter
AU - Gustafsson, Jan åke
AU - Larsson, Agne
AU - Skett, Paul
AU - Stenberg, åke
AU - Sonnenschein, Carlos
N1 - Funding Information:
This work was supported by grants from the Swedish Medical Research Council, Harald and Greta Jeanssons Stiftelse, WHO, and the USPHS. P. S. is a fellow of the CIBA-GEIGY Fellowship Trust. C. S. is a USPHS Research Career Development awardee.
PY - 1976/3
Y1 - 1976/3
N2 - After transplantation of MIT/F4 pituitary tumor cells to male rats of the Fisher strain, the masculine type of hepatic steroid metabolism was changed into a feminine pattern of enzyme activities. Liver metabolism of steroid hormones in female rats was relatively unaffected following transplantation of pituitary tumor cells. Furthermore, extract from MIT/F4 tumors and "autonomous" pituitary tissue increased the 5α-reductase activity of hepatoma cells in the culture (HTC cells) at subsaturation concentrations of the substrate 4-androstene-3, 17-dione by decreasing the apparent Km of the enzyme. It is concluded that the pituitary tumor (in accord with the secretion from an "autonomous" pituitary gland) secretes "feminotropin," a novel hypophyseal principle that probably is an important regulator of hepatic steroid metabolism. It is suggested that pituitary tumor tissue of the MtT/F4 type could be used as source of feminotropin in purification studies.
AB - After transplantation of MIT/F4 pituitary tumor cells to male rats of the Fisher strain, the masculine type of hepatic steroid metabolism was changed into a feminine pattern of enzyme activities. Liver metabolism of steroid hormones in female rats was relatively unaffected following transplantation of pituitary tumor cells. Furthermore, extract from MIT/F4 tumors and "autonomous" pituitary tissue increased the 5α-reductase activity of hepatoma cells in the culture (HTC cells) at subsaturation concentrations of the substrate 4-androstene-3, 17-dione by decreasing the apparent Km of the enzyme. It is concluded that the pituitary tumor (in accord with the secretion from an "autonomous" pituitary gland) secretes "feminotropin," a novel hypophyseal principle that probably is an important regulator of hepatic steroid metabolism. It is suggested that pituitary tumor tissue of the MtT/F4 type could be used as source of feminotropin in purification studies.
UR - https://www.scopus.com/pages/publications/0017283416
UR - https://www.scopus.com/inward/citedby.url?scp=0017283416&partnerID=8YFLogxK
U2 - 10.1016/0092-8674(76)90171-9
DO - 10.1016/0092-8674(76)90171-9
M3 - Article
C2 - 947549
AN - SCOPUS:0017283416
SN - 0092-8674
VL - 7
SP - 413
EP - 417
JO - Cell
JF - Cell
IS - 3
ER -