TY - JOUR
T1 - Female Pattern of Metabolism of [4-14C]Corticosterone in Male Pseudohermaphroditic Rats
AU - Goldman, Allen S.
AU - Gustafsson, Jan Ake
AU - Gustafsson, Sven A.
N1 - Funding Information:
Recipient of Career Development Award (HD-13,682) from the U.S. Public Health Service.
PY - 1973/2
Y1 - 1973/2
N2 - The metabolites of [4-14C]corticosterone appearing in bile fistulae of male pseudohermaphrodites and littermate male and female Stanley-Gumbreck rats were determined by gas-liquid chromatography, radio-gas chromatography and gas chromatog-raphy-mass spectrometry. Male pseudohermaphroditic rats excrete corticosterone metabolites identical to those in bile from female rats: 3 a,11 β,21-trihydroxy-5 a -pregnan-20-one and 3 a, 11β, 15 a,21-tetrahydroxy-5 a -pregnan-20-one in the monosulfate fraction and, and 3 a, 11β,21-trihydroxy-5 a -pregnan-20-one, 3 a, 11β, 15 a,21-tetrahydroxy-5 a -pregnan-20-one, and 3β,11β,21-trihydroxy-5 a -pregnan-20-one in the disulfate fraction. Male rats excrete 5 a -pregnane-3β,11β,20β,21-tetrol in the monosulfate fraction and 3β,11β,21-trihydroxy-5 a -pregnan-20-one and 5 a -pregnane-3β,11β,20β,21-tetrol in the disulfate fraction. The female type of corticosterone metabolism in the male pseudohermaphroditic rat is probably due to the lack in these animals of a postnatal testosterone imprinting effect on the liver combined with hepatic insensitivity to testosterone in adult life. Large doses of testosterone propionate completely suppress the excretion of 3 a,11β,15 a,21-tetrahydroxy-5 a -pregnan-20-one in bile from a pseudoher-maphroditic rat indicating a relative rather than an absolute testosterone insensitivity of the liver of these rats. The authors are grateful to Drs. A. J. Stanley, J. E. Allison, and L. G. Gumbreck for supplying the male pseudohermaphroditic and littermate rats.
AB - The metabolites of [4-14C]corticosterone appearing in bile fistulae of male pseudohermaphrodites and littermate male and female Stanley-Gumbreck rats were determined by gas-liquid chromatography, radio-gas chromatography and gas chromatog-raphy-mass spectrometry. Male pseudohermaphroditic rats excrete corticosterone metabolites identical to those in bile from female rats: 3 a,11 β,21-trihydroxy-5 a -pregnan-20-one and 3 a, 11β, 15 a,21-tetrahydroxy-5 a -pregnan-20-one in the monosulfate fraction and, and 3 a, 11β,21-trihydroxy-5 a -pregnan-20-one, 3 a, 11β, 15 a,21-tetrahydroxy-5 a -pregnan-20-one, and 3β,11β,21-trihydroxy-5 a -pregnan-20-one in the disulfate fraction. Male rats excrete 5 a -pregnane-3β,11β,20β,21-tetrol in the monosulfate fraction and 3β,11β,21-trihydroxy-5 a -pregnan-20-one and 5 a -pregnane-3β,11β,20β,21-tetrol in the disulfate fraction. The female type of corticosterone metabolism in the male pseudohermaphroditic rat is probably due to the lack in these animals of a postnatal testosterone imprinting effect on the liver combined with hepatic insensitivity to testosterone in adult life. Large doses of testosterone propionate completely suppress the excretion of 3 a,11β,15 a,21-tetrahydroxy-5 a -pregnan-20-one in bile from a pseudoher-maphroditic rat indicating a relative rather than an absolute testosterone insensitivity of the liver of these rats. The authors are grateful to Drs. A. J. Stanley, J. E. Allison, and L. G. Gumbreck for supplying the male pseudohermaphroditic and littermate rats.
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U2 - 10.3181/00379727-142-37096
DO - 10.3181/00379727-142-37096
M3 - Article
C2 - 4689407
AN - SCOPUS:0015576097
SN - 0037-9727
VL - 142
SP - 691
EP - 696
JO - Proceedings of the Society for Experimental Biology and Medicine
JF - Proceedings of the Society for Experimental Biology and Medicine
IS - 2
ER -