Fatty acid composition of lipids which copurify with band 3

Leonard R. Maneri, Philip Low

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

In a previous study (L. R. Maneri and P. S. Low (1988) J. Biol. Chem.263, 16170-16178) we determined that the anion transport protein, band 3, was significantly stabilized by lipids containing saturated and/or long chain fatty acids. To determine whether this thermodynamic preference is reflected in the composition of lipids tightly associating with the anion transporter invivo, we have analyzed the fatty acid content of phospholipids co-isolating with the purified integral domain of band 3. Our data demonstrate that although stearic acid comprises only 14% of the bulk lipid fatty acids of the red cell membrane, it constitutes ∼68% of the fatty acids of lipids co-isolating with band 3. Certain other long chain fatty acids were also enriched in the adherent lipids. These results suggest that the fatty acids which most effectively stabilize band 3 also have the highest affinity for the transport protein.

Original languageEnglish (US)
Pages (from-to)1012-1019
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume159
Issue number3
DOIs
StatePublished - Mar 31 1989

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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