TY - JOUR
T1 - Fasting time duration modulates the onset of insulin-induced hypoglycemic seizures in mice
AU - Pitchaimani, Vigneshwaran
AU - Arumugam, Somasundaram
AU - Thandavarayan, Rajarajan Amirthalingam
AU - Karuppagounder, Vengadeshprabhu
AU - Afrin, Mst Rejina
AU - Sreedhar, Remya
AU - Harima, Meilei
AU - Suzuki, Hiroshi
AU - Miyashita, Shizuka
AU - Nakamura, Takashi
AU - Suzuki, Kenji
AU - Nakamura, Masahiko
AU - Ueno, Kazuyuki
AU - Watanabe, Kenichi
N1 - Funding Information:
This research was supported by Ministry of Education, Culture, Sports, Sciences and Technology, Japan and by a grant from the promotion and mutual aid corporation for private schools, Japan (23602012 and 26460239) respectively.
Publisher Copyright:
© 2016 Elsevier B.V.
PY - 2016/9/1
Y1 - 2016/9/1
N2 - Objective Fasting (48 h) in mice causes resistance to insulin-induced hypoglycemic seizures (IIHS) but in rats fasting (14–16 h) predisposes IIHS. So we suspect the duration of fasting may possibly affect the onset of seizures and in this study, we investigated the IIHS by administering 8 Units (U) insulin (INS)/k.g., intraperitoneally to 8 weeks old male C57BL6/J mice. Methods The mice were divided into group 1 (non-fasted), group 2 (6 h fasted) and group 3 (24 h fasted) and we administered the 8 U INS. The first behavioral hypoglycemic seizure symptoms such as jump, clonus or barrel rotations considered as seizure onset and we analyzed the blood glucose level (BGL) and serum beta-hydroxybutyrate (BHB) level. Results The time of first seizure onset in group 1 was 109.7 ± 4.3 min, group 2 was 46.50 ± 3.9 min and group 3 was 165.4 ± 13.26 min. The seizure onset time in group 2 was significantly decreased compared to group 1. The seizure onset time in group 3 was significantly increased compared to group 1 and group 2. The decreased BGL after INS administration was correlated with the seizure onset time in group 1 and group 2 but not in group 3. The BHB level in group 3 was significantly higher compared to group 1 and 2. Conclusion Our data show that the fasting time duration significantly modulates the onset of hypoglycemic seizures. The opposite effect of 6 h or 24 h fasting time duration is likely caused by different BHB levels.
AB - Objective Fasting (48 h) in mice causes resistance to insulin-induced hypoglycemic seizures (IIHS) but in rats fasting (14–16 h) predisposes IIHS. So we suspect the duration of fasting may possibly affect the onset of seizures and in this study, we investigated the IIHS by administering 8 Units (U) insulin (INS)/k.g., intraperitoneally to 8 weeks old male C57BL6/J mice. Methods The mice were divided into group 1 (non-fasted), group 2 (6 h fasted) and group 3 (24 h fasted) and we administered the 8 U INS. The first behavioral hypoglycemic seizure symptoms such as jump, clonus or barrel rotations considered as seizure onset and we analyzed the blood glucose level (BGL) and serum beta-hydroxybutyrate (BHB) level. Results The time of first seizure onset in group 1 was 109.7 ± 4.3 min, group 2 was 46.50 ± 3.9 min and group 3 was 165.4 ± 13.26 min. The seizure onset time in group 2 was significantly decreased compared to group 1. The seizure onset time in group 3 was significantly increased compared to group 1 and group 2. The decreased BGL after INS administration was correlated with the seizure onset time in group 1 and group 2 but not in group 3. The BHB level in group 3 was significantly higher compared to group 1 and 2. Conclusion Our data show that the fasting time duration significantly modulates the onset of hypoglycemic seizures. The opposite effect of 6 h or 24 h fasting time duration is likely caused by different BHB levels.
KW - Fasting
KW - Hypoglycemic autonomic failure
KW - Hypoglycemic seizures
KW - Ketone bodies
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U2 - 10.1016/j.eplepsyres.2016.06.009
DO - 10.1016/j.eplepsyres.2016.06.009
M3 - Article
C2 - 27392286
AN - SCOPUS:84978116634
SN - 0920-1211
VL - 125
SP - 47
EP - 51
JO - Epilepsy Research
JF - Epilepsy Research
ER -