Fasting time duration modulates the onset of insulin-induced hypoglycemic seizures in mice

Vigneshwaran Pitchaimani, Somasundaram Arumugam, Rajarajan Amirthalingam Thandavarayan, Vengadeshprabhu Karuppagounder, Mst Rejina Afrin, Remya Sreedhar, Meilei Harima, Hiroshi Suzuki, Shizuka Miyashita, Takashi Nakamura, Kenji Suzuki, Masahiko Nakamura, Kazuyuki Ueno, Kenichi Watanabe

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


Objective Fasting (48 h) in mice causes resistance to insulin-induced hypoglycemic seizures (IIHS) but in rats fasting (14–16 h) predisposes IIHS. So we suspect the duration of fasting may possibly affect the onset of seizures and in this study, we investigated the IIHS by administering 8 Units (U) insulin (INS)/k.g., intraperitoneally to 8 weeks old male C57BL6/J mice. Methods The mice were divided into group 1 (non-fasted), group 2 (6 h fasted) and group 3 (24 h fasted) and we administered the 8 U INS. The first behavioral hypoglycemic seizure symptoms such as jump, clonus or barrel rotations considered as seizure onset and we analyzed the blood glucose level (BGL) and serum beta-hydroxybutyrate (BHB) level. Results The time of first seizure onset in group 1 was 109.7 ± 4.3 min, group 2 was 46.50 ± 3.9 min and group 3 was 165.4 ± 13.26 min. The seizure onset time in group 2 was significantly decreased compared to group 1. The seizure onset time in group 3 was significantly increased compared to group 1 and group 2. The decreased BGL after INS administration was correlated with the seizure onset time in group 1 and group 2 but not in group 3. The BHB level in group 3 was significantly higher compared to group 1 and 2. Conclusion Our data show that the fasting time duration significantly modulates the onset of hypoglycemic seizures. The opposite effect of 6 h or 24 h fasting time duration is likely caused by different BHB levels.

Original languageEnglish (US)
Pages (from-to)47-51
Number of pages5
JournalEpilepsy Research
StatePublished - Sep 1 2016


  • Fasting
  • Hypoglycemic autonomic failure
  • Hypoglycemic seizures
  • Ketone bodies

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology


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