False negative factor V Leiden assay following allogeneic stem cell transplant

Kendall P. Crookston, Ruth Henderson, Wayne L. Chandler

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

We report a case where a phenotypic test (an activity assay for activated protein C resistance) correctly indicated that the patient had an abnormality, whereas the initial genetic test (a PCR-based DNA-assay used to detect the mutation in the FV gene) incorrectly indicated that the patient did not. The apparent false negative result of the DNA-based test was due to the use of peripheral blood leucocytes for DNA analysis. The patient had undergone a stem cells transplant several months before, and the leucocytes in her blood were derived from the item cell donor, which lacked the FV defect.

Original languageEnglish (US)
Pages (from-to)600-602
Number of pages3
JournalBritish Journal of Haematology
Volume100
Issue number3
DOIs
StatePublished - 1998

Keywords

  • Activated protein C resistance
  • Factor V Leiden
  • Thrombophilia
  • Thrombosis
  • Transplantation

ASJC Scopus subject areas

  • Hematology

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