False negative factor V Leiden assay following allogeneic stem cell transplant

Kendall P. Crookston, Ruth Henderson, Wayne L. Chandler

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


We report a case where a phenotypic test (an activity assay for activated protein C resistance) correctly indicated that the patient had an abnormality, whereas the initial genetic test (a PCR-based DNA-assay used to detect the mutation in the FV gene) incorrectly indicated that the patient did not. The apparent false negative result of the DNA-based test was due to the use of peripheral blood leucocytes for DNA analysis. The patient had undergone a stem cells transplant several months before, and the leucocytes in her blood were derived from the item cell donor, which lacked the FV defect.

Original languageEnglish (US)
Pages (from-to)600-602
Number of pages3
JournalBritish Journal of Haematology
Issue number3
StatePublished - 1998


  • Activated protein C resistance
  • Factor V Leiden
  • Thrombophilia
  • Thrombosis
  • Transplantation

ASJC Scopus subject areas

  • Hematology


Dive into the research topics of 'False negative factor V Leiden assay following allogeneic stem cell transplant'. Together they form a unique fingerprint.

Cite this