TY - JOUR
T1 - Failure of Chloro-s-triazine-Derived Compounds to Induce Estrogenic Responses in Vivo and in Vitro
AU - Connor, K.
AU - Howell, J.
AU - Safe, S.
AU - Chen, I.
AU - Liu, H.
AU - Berhane, K.
AU - Sciarretta, C.
AU - Zacharewski, T.
PY - 1998
Y1 - 1998
N2 - The estrogenic activity of simazine and atrazine was investigated in the immature female Sprague-Dawley rat uterus, the estrogen-responsive MCF-7 human breast cancer cell line and PL3 yeast strain. Atrazine and simazine (50 - 300 mg/kg × 3) did not induce rat uterine wet weight, cytosolic progesterone receptor (PR) levels, or uterine peroxidase activity. In rats cotreated with 17β-estradiol (E2) plus atrazine or simazine, there was some inhibition of E2-induced uterine PR binding and peroxidase activity. In MCF-7 cells, simazine and atrazine did not affect E2-induced cell proliferation, nuclear PR levels or luciferase activity in cells transiently transfected with the Gal4-estrogen receptor chimera and a Gal4-regulated luciferase reporter gene, and no antiestrogenic activity was observed in cotreated cells, while growth was observed on similar media supplemented with 1 nM E2. These results indicate that atrazine and simazine are not estrogenic.
AB - The estrogenic activity of simazine and atrazine was investigated in the immature female Sprague-Dawley rat uterus, the estrogen-responsive MCF-7 human breast cancer cell line and PL3 yeast strain. Atrazine and simazine (50 - 300 mg/kg × 3) did not induce rat uterine wet weight, cytosolic progesterone receptor (PR) levels, or uterine peroxidase activity. In rats cotreated with 17β-estradiol (E2) plus atrazine or simazine, there was some inhibition of E2-induced uterine PR binding and peroxidase activity. In MCF-7 cells, simazine and atrazine did not affect E2-induced cell proliferation, nuclear PR levels or luciferase activity in cells transiently transfected with the Gal4-estrogen receptor chimera and a Gal4-regulated luciferase reporter gene, and no antiestrogenic activity was observed in cotreated cells, while growth was observed on similar media supplemented with 1 nM E2. These results indicate that atrazine and simazine are not estrogenic.
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U2 - 10.1021/bk-1998-0683.ch033
DO - 10.1021/bk-1998-0683.ch033
M3 - Article
AN - SCOPUS:0347653522
VL - 683
SP - 424
EP - 431
JO - ACS Symposium Series
JF - ACS Symposium Series
SN - 0097-6156
ER -