Facile approach to observe and quantify the αiIbβ3 Integrin on a Single-Cell

Jiao Zhai, Yaling Wang, Chao Xu, Lingna Zheng, Meng Wang, Weiyue Feng, Liang Gao, Lina Zhao, Ru Liu, Fuping Gao, Yuliang Zhao, Zhifang Chai, Xueyun Gao

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

We report for the first time seeing and counting integrin αIIbβ3 on a single-cell level. The proposed method is based on the using of the Au cluster probe. With the fluorescent property of Au24 cluster and the specific targeting ability of peptide, our probe can directly visualize integrin αIIbβ3 on the membrane of human erythroleukemia cells (HEL) via confocal microscopy. On the basis of the accurate formula of our probe (Au24Peptide8), the number of integrin αIIbβ3 can be precisely counted by quantifying the gold content on a single HEL cell via laser ablation inductively coupled plasma mass spectrometry. Our results reveal that the number of integrin αIIbβ3 on a single cell varies from 5.75 × 10-17 to 9.11 × 10-17 mol, because of the heteroexpression levels of αIIbβ3 on individual cells.

Original languageEnglish (US)
Pages (from-to)2546-2549
Number of pages4
JournalAnalytical Chemistry
Volume87
Issue number5
DOIs
StatePublished - Mar 3 2015

ASJC Scopus subject areas

  • Analytical Chemistry

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