TY - JOUR
T1 - Fabrication of innovative silk/alginate microcarriers for mesenchymal stem cell delivery and tissue regeneration
AU - Perteghella, Sara
AU - Martella, Elisa
AU - De Girolamo, Laura
AU - Orfei, Carlotta Perucca
AU - Pierini, Michela
AU - Fumagalli, Valentina
AU - Pintacuda, Domenica Valeria
AU - Chlapanidas, Theodora
AU - Viganò, Marco
AU - Faragò, Silvio
AU - Torre, Maria Luisa
AU - Lucarelli, Enrico
N1 - Funding Information:
Acknowledgments: The work was supported by Regione Lombardia (STEMDELIVERY, Project ID. 42617604). The authors thank Nembri Industrie Tessili S.r.l. (Capriolo, Italy) for Bombyx mori cocoons, Giovanna Bruni (University of Pavia, Italy) for willingness on the use of Mastersizer 2000 (Malvern, Worcestershire, UK), Vittorio Necchi (Centro Grandi Strumenti, Pavia University, Italy) for the ultrastructural investigation, Spartaco Santi (Centro Nazionale delle Ricerche, Bologna, Italy) for the technical support with confocal imaging, Mariapia Cumani (Rizzoli Institute, Bologna) for designing the figure graphics, and Ryan Rogers, University of Michigan, for editorial help.
Publisher Copyright:
© 2017 by the authors. Licensee MDPI, Basel, Switzerland.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2017/9
Y1 - 2017/9
N2 - The aim of this study was to exploit silk fibroin’s properties to develop innovative composite microcarriers for mesenchymal stem cell (MSCs) adhesion and proliferation. Alginate microcarriers were prepared, added to silk fibroin solution, and then treated with ethanol to induce silk conformational transition. Microcarriers were characterized for size distribution, coating stability and homogeneity. Finally, in vitro cytocompatibility and suitability as delivery systems for MSCs were investigated. Results indicated that our manufacturing process is consistent and reproducible: silk/alginate microcarriers were stable, with spherical geometry, about 400 μm in average diameter, and fibroin homogeneously coated the surface. MSCs were able to adhere rapidly onto the microcarrier surface and to cover the surface of the microcarrier within three days of culture, moreover, on this innovative 3D culture system, stem cells preserved their metabolic activity and their multi-lineage differentiation potential. In conclusion, silk/alginate microcarriers represent a suitable support for MSCs culture and expansion. Since it is able to preserve MSCs multipotency, the developed 3D system can be intended for cell delivery, for advanced therapy and regenerative medicine applications.
AB - The aim of this study was to exploit silk fibroin’s properties to develop innovative composite microcarriers for mesenchymal stem cell (MSCs) adhesion and proliferation. Alginate microcarriers were prepared, added to silk fibroin solution, and then treated with ethanol to induce silk conformational transition. Microcarriers were characterized for size distribution, coating stability and homogeneity. Finally, in vitro cytocompatibility and suitability as delivery systems for MSCs were investigated. Results indicated that our manufacturing process is consistent and reproducible: silk/alginate microcarriers were stable, with spherical geometry, about 400 μm in average diameter, and fibroin homogeneously coated the surface. MSCs were able to adhere rapidly onto the microcarrier surface and to cover the surface of the microcarrier within three days of culture, moreover, on this innovative 3D culture system, stem cells preserved their metabolic activity and their multi-lineage differentiation potential. In conclusion, silk/alginate microcarriers represent a suitable support for MSCs culture and expansion. Since it is able to preserve MSCs multipotency, the developed 3D system can be intended for cell delivery, for advanced therapy and regenerative medicine applications.
KW - Alginate
KW - Mesenchymal stem cells
KW - Microcarriers
KW - Musculoskeletal tissues
KW - Regenerative medicine
KW - Silk fibroin
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U2 - 10.3390/ijms18091829
DO - 10.3390/ijms18091829
M3 - Article
C2 - 28832547
AN - SCOPUS:85028309536
VL - 18
JO - International journal of molecular sciences
JF - International journal of molecular sciences
SN - 1661-6596
IS - 9
M1 - 1829
ER -