Fabrication and pilot in vivo study of a collagen-BDDGE-elastin core-shell scaffold for tendon regeneration

Monica Sandri, Giuseppe Filardo, Elizaveta Kon, Silvia Panseri, Monica Montesi, Michele Iafisco, Elisa Savini, Simone Sprio, Carla Cunha, Gianluca Giavaresi, Francesca Veronesi, Milena Fini, Luca Salvatore, Alessandro Sannino, Maurilio Marcacci, Anna Tampieri

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


The development of bio-devices for complete regeneration of ligament and tendon tissues is presently one of the biggest challenges in tissue engineering. Such device must simultaneously possess optimal mechanical performance, suitable porous structure, and biocompatible microenvironment. This study proposes a novel collagen-BDDGE-elastin (CBE)-based device for tendon tissue engineering, by the combination of two different modules: (i) a load-bearing, non-porous, "core scaffold" developed by braiding CBE membranes fabricated via an evaporative process and (ii) a hollow, highly porous, "shell scaffold" obtained by uniaxial freezing followed by freeze-drying of CBE suspension, designed to function as a physical guide and reservoir of cells to promote the regenerative process. Both core and shell materials demonstrated good cytocompatibility in vitro, and notably, the porous shell architecture directed cell alignment and population within the sample. Finally, a prototype of the core module was implanted in a rat tendon lesion model, and histological analysis demonstrated its safety, biocompatibility, and ability to induce tendon regeneration. Overall, our results indicate that such device may have the potential to support and induce in situ tendon regeneration.

Original languageEnglish (US)
Article number52
JournalFrontiers in Bioengineering and Biotechnology
Issue numberJUN
StatePublished - Jun 28 2016


  • Biomimetic scaffold
  • Collagen
  • Elastin
  • Ligament
  • Tendon
  • Tissue regeneration

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Histology
  • Biomedical Engineering


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