Extraction and purification of genomic DNA via entrapment in an array of microposts

Juraj Topolancik, David R. Latulippe, Harvey Tian, Christopher Wallin, Harold G. Craighead

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A novel microfluidic device was developed to extract and purify genomic DNA from a small number (from a single to hundreds) of cells using an array of microposts. Following chemical-induced lysis, separation occurs when large genomic DNA strands became physically entrapped within the post array while other smaller cellular components were flushed out of the device. The purified DNA was extracted by a continuous flow enzymatic digestion process which fragmented the trapped DNA into smaller sizes. Using this strategy, we have obtained high DNA extraction efficiencies (~ 85 to 90%) for low elution volumes (≤ 20 μL).

Original languageEnglish (US)
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages1026-1028
Number of pages3
Volume2
StatePublished - Dec 1 2011
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: Oct 2 2011Oct 6 2011

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period10/2/1110/6/11

Keywords

  • DNA entrapment
  • DNA extraction
  • Micropost array

ASJC Scopus subject areas

  • Control and Systems Engineering

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