Abstract
Monocyte-induced endothelial cell inflammation is associated with multiple pathological conditions, and extracellular vesicles (EVs) are essential nanosized components of intercellular communication. EVs derived from endotoxin-stimulated monocytes were previously shown to carry pro-inflammatory proteins and RNAs. The role of glucose transporter-1 (GLUT-1) and glycan features in monocyte-derived EV-induced endothelial cell inflammation remains largely unexplored. This study demonstrates that EVs derived from endotoxin-stimulated monocytes activate inflammatory pathways in endothelial cells, which are partially attributed to GLUT-1. Alterations in glycan features and increased levels of GLUT-1 were observed in EVs derived from endotoxin-stimulated monocytes. Notably, inhibition of EV-associated GLUT-1, through the use of fasentin, suppressed EV-induced inflammatory cytokines in recipient endothelial cells.
Original language | English (US) |
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Article number | 102515 |
Pages (from-to) | 102515 |
Journal | Nanomedicine: Nanotechnology, Biology, and Medicine |
Volume | 42 |
DOIs | |
State | Published - Jun 2022 |
Keywords
- Exosome
- Extracellular vesicle
- Glucose transporter-1
- Glycan node analysis
- Inflammation
- Endotoxins/pharmacology
- Humans
- Inflammation/metabolism
- Polysaccharides/metabolism
- Extracellular Vesicles/metabolism
- Endothelial Cells/metabolism
- Glucose Transporter Type 1/metabolism
- Monocytes/metabolism
ASJC Scopus subject areas
- Bioengineering
- Molecular Medicine
- General Materials Science
- Biomedical Engineering
- Medicine (miscellaneous)
- Pharmaceutical Science