Extracellular recordings from patterned neuronal networks using planar microelectrode arrays

Conrad D. James, Andrew J H Spence, Natalie M. Dowell-Mesfin, Rifat J. Hussain, Karen L. Smith, Harold G. Craighead, Michael S. Isaacson, William Shain, James N. Turner

Research output: Contribution to journalArticle

106 Scopus citations

Abstract

Neuronal cell networks have been reconstructed on planar microelectrode arrays (MEAs) from dissociated hippocampal pyramidal neurons. Microcontact printing (μCP) and a photoresist-liftoff method were used to selectively localize poly-L-lysine (PLL) on the surface of MEAs. Haptotaxis led to the organization of the neurons into networks localized adjacent to microelectrodes. Various grids of PLL with 2-25-μm-wide lines spaced by 50-200 μm with 15-25-μm nodes at intersection points were used to guide cell body attachment and neurite outgrowth. Bursting activity with spike amplitude attenuation was observed, and multichannel recordings detected instances of coincident firing activity. Finally, we present here an extracellular recording from a ∼2 μm bundle of guided neurites.

Original languageEnglish (US)
Pages (from-to)1640-1648
Number of pages9
JournalIEEE Transactions on Biomedical Engineering
Volume51
Issue number9
DOIs
StatePublished - Sep 2004

Keywords

  • Hippocampal pyramidal neuron
  • Microcontact printing
  • Microelectrode array
  • Neuron networks

ASJC Scopus subject areas

  • Biomedical Engineering

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